Development of serological assays to identify<i>Helicobacter suis</i>and<i>Helicobacter pylori</i>infections
SUMMARY Helicobacter suishosted by hogs is the most prevalent gastric non-Helicobacter pylori Helicobacterspecies found in humans. Recent studies suggest that theH. suisinfection has already induced many cases of gastric disease. However, the infection period and route ofH. suisfrom hogs remain unclear. Because diagnostic methods based on the urease activity ofH. suisoften yield negative judgments, there is no reliable method for diagnosingH. suisinfection in clinical practice without gastric biopsy specimens. We developed the world’s first ELISA to simultaneously diagnoseH. suisandH. pyloriinfection in a single test. The area under the ROC curve was 0.9648 or 0.9200 for identifyingH. suisorH. pyloriinfection, respectively. The sensitivity, specificity, and positive and negative predictive values for identifyingH. suisinfection were 100%, 92.6%, 76.9%, and 100%, and those for identifyingH. pyloriinfection were 88.2%, 87.5%, 65.2%, and 96.6%, respectively. (150 words).
| Medienart: |
|
|---|
Preprint| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
bioRxiv.org - (2023) vom: 28. Nov. Zur Gesamtaufnahme - year:2023 |
|---|
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Matsui, Hidenori [VerfasserIn] |
|---|
| Links: |
Volltext [kostenfrei] |
|---|
| Themen: |
|---|
| doi: |
10.1101/2022.10.10.22280809 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
XBI037552597 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | XBI037552597 | ||
| 003 | DE-627 | ||
| 005 | 20231205145921.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 221013s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1101/2022.10.10.22280809 |2 doi | |
| 035 | |a (DE-627)XBI037552597 | ||
| 035 | |a (biorXiv)10.1101/2022.10.10.22280809 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Matsui, Hidenori |e verfasserin |0 (orcid)0000-0001-7791-2356 |4 aut | |
| 245 | 1 | 0 | |a Development of serological assays to identify<i>Helicobacter suis</i>and<i>Helicobacter pylori</i>infections |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a SUMMARY Helicobacter suishosted by hogs is the most prevalent gastric non-Helicobacter pylori Helicobacterspecies found in humans. Recent studies suggest that theH. suisinfection has already induced many cases of gastric disease. However, the infection period and route ofH. suisfrom hogs remain unclear. Because diagnostic methods based on the urease activity ofH. suisoften yield negative judgments, there is no reliable method for diagnosingH. suisinfection in clinical practice without gastric biopsy specimens. We developed the world’s first ELISA to simultaneously diagnoseH. suisandH. pyloriinfection in a single test. The area under the ROC curve was 0.9648 or 0.9200 for identifyingH. suisorH. pyloriinfection, respectively. The sensitivity, specificity, and positive and negative predictive values for identifyingH. suisinfection were 100%, 92.6%, 76.9%, and 100%, and those for identifyingH. pyloriinfection were 88.2%, 87.5%, 65.2%, and 96.6%, respectively. (150 words) | ||
| 650 | 4 | |a Biology |7 (dpeaa)DE-84 | |
| 650 | 4 | |a 570 |7 (dpeaa)DE-84 | |
| 700 | 1 | |a Rimbara, Emiko |4 aut | |
| 700 | 1 | |a Suzuki, Masato |4 aut | |
| 700 | 1 | |a Tokunaga, Kengo |4 aut | |
| 700 | 1 | |a Suzuki, Hidekazu |4 aut | |
| 700 | 1 | |a Sano, Masaya |4 aut | |
| 700 | 1 | |a Ueda, Takashi |4 aut | |
| 700 | 1 | |a Tsugawa, Hitoshi |4 aut | |
| 700 | 1 | |a Nanjo, Sohachi |4 aut | |
| 700 | 1 | |a Takeda, Akira |4 aut | |
| 700 | 1 | |a Sasaki, Makoto |4 aut | |
| 700 | 1 | |a Terao, Shuichi |4 aut | |
| 700 | 1 | |a Suda, Tsuyoshi |4 aut | |
| 700 | 1 | |a Aoki, Sae |4 aut | |
| 700 | 1 | |a Shibayama, Keigo |4 aut | |
| 700 | 1 | |a Ota, Hiroyoshi |4 aut | |
| 700 | 1 | |a Mabe, Katsuhiro |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t bioRxiv.org |g (2023) vom: 28. Nov. |
| 773 | 1 | 8 | |g year:2023 |g day:28 |g month:11 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1101/2022.10.10.22280809 |z kostenfrei |3 Volltext |
| 912 | |a GBV_XBI | ||
| 951 | |a AR | ||
| 952 | |j 2023 |b 28 |c 11 | ||