Native doublet microtubules from<i>Tetrahymena thermophila</i>reveal the importance of outer junction proteins
Abstract Cilia are ubiquitous eukaryotic organelles responsible for cellular motility and sensory functions. The ciliary axoneme is a microtubule-based cytoskeleton consisting of two central singlets and nine outer doublet microtubules. Cryo-electron microscopy-based studies have revealed a complex network inside the lumen of both tubules composed of microtubule-inner proteins (MIPs). However, the functions of most MIPs remain unknown. Here, we present single-particle cryo-EM-based analyses of theTetrahymena thermophilanative doublet microtubule and identify 38 MIPs. These data shed light on the evolutionarily conserved and diversified roles of MIPs. In addition, we identified MIPs potentially responsible for the assembly and stability of the doublet outer junction. Knockout of the evolutionarily conserved outer junction component CFAP77 moderately diminishesTetrahymenaswimming speed and beat frequency, indicating the important role of CFAP77 and outer junction stability in cilia beating generation and/or regulation..
| Medienart: |
|
|---|
Preprint| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
bioRxiv.org - (2024) vom: 23. Apr. Zur Gesamtaufnahme - year:2024 |
|---|
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Kubo, Shintaroh [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
|---|
| doi: |
10.1101/2022.09.30.510376 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
XBI037447556 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | XBI037447556 | ||
| 003 | DE-627 | ||
| 005 | 20240425104449.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 221005s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1101/2022.09.30.510376 |2 doi | |
| 035 | |a (DE-627)XBI037447556 | ||
| 035 | |a (biorXiv)10.1101/2022.09.30.510376 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Kubo, Shintaroh |e verfasserin |0 (orcid)0000-0002-0946-8879 |4 aut | |
| 245 | 1 | 0 | |a Native doublet microtubules from<i>Tetrahymena thermophila</i>reveal the importance of outer junction proteins |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a Abstract Cilia are ubiquitous eukaryotic organelles responsible for cellular motility and sensory functions. The ciliary axoneme is a microtubule-based cytoskeleton consisting of two central singlets and nine outer doublet microtubules. Cryo-electron microscopy-based studies have revealed a complex network inside the lumen of both tubules composed of microtubule-inner proteins (MIPs). However, the functions of most MIPs remain unknown. Here, we present single-particle cryo-EM-based analyses of theTetrahymena thermophilanative doublet microtubule and identify 38 MIPs. These data shed light on the evolutionarily conserved and diversified roles of MIPs. In addition, we identified MIPs potentially responsible for the assembly and stability of the doublet outer junction. Knockout of the evolutionarily conserved outer junction component CFAP77 moderately diminishesTetrahymenaswimming speed and beat frequency, indicating the important role of CFAP77 and outer junction stability in cilia beating generation and/or regulation. | ||
| 650 | 4 | |a Biology |7 (dpeaa)DE-84 | |
| 650 | 4 | |a 570 |7 (dpeaa)DE-84 | |
| 700 | 1 | |a Black, Corbin |e verfasserin |0 (orcid)0000-0003-2777-6434 |4 aut | |
| 700 | 1 | |a Joachimiak, Ewa |e verfasserin |0 (orcid)0000-0003-3185-7923 |4 aut | |
| 700 | 1 | |a Yang, Shun Kai |e verfasserin |0 (orcid)0000-0002-2363-1441 |4 aut | |
| 700 | 1 | |a Legal, Thibault |e verfasserin |0 (orcid)0000-0003-1477-1722 |4 aut | |
| 700 | 1 | |a Peri, Katya |e verfasserin |0 (orcid)0000-0002-7367-7501 |4 aut | |
| 700 | 1 | |a Khalifa, Ahmad |e verfasserin |0 (orcid)0000-0001-5569-2014 |4 aut | |
| 700 | 1 | |a Ghanaeian, Avrin |e verfasserin |4 aut | |
| 700 | 1 | |a Valente-Paterno, Melissa |e verfasserin |0 (orcid)0000-0001-8470-3739 |4 aut | |
| 700 | 1 | |a De Bellis, Chelsea |e verfasserin |4 aut | |
| 700 | 1 | |a Huynh, Phuong |e verfasserin |4 aut | |
| 700 | 1 | |a Fan, Zhe |e verfasserin |4 aut | |
| 700 | 1 | |a Wloga, Dorota |e verfasserin |0 (orcid)0000-0003-3314-7773 |4 aut | |
| 700 | 1 | |a Bui, Khanh Huy |e verfasserin |0 (orcid)0000-0003-2814-9889 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t bioRxiv.org |g (2024) vom: 23. Apr. |
| 773 | 1 | 8 | |g year:2024 |g day:23 |g month:04 |
| 856 | 4 | 0 | |u https://doi.org/10.1038/s41467-023-37868-0 |x 0 |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1101/2022.09.30.510376 |x 0 |z kostenfrei |3 Volltext |
| 912 | |a GBV_XBI | ||
| 951 | |a AR | ||
| 952 | |j 2024 |b 23 |c 04 | ||