Repression of divergent noncoding transcription by a sequence-specific transcription factor
Summary Many active eukaryotic gene promoters exhibit divergent noncoding transcription, but the mechanisms restricting expression of these transcripts are not well understood. Here we demonstrate how a sequence-specific transcription factor represses divergent noncoding transcription at highly expressed genes in yeast. We find that depletion of the transcription factor Rap1 induces noncoding transcription in a large fraction of Rap1 regulated gene promoters. Specifically, Rap1 prevents transcription initiation at cryptic promoters near its binding sites, which is uncoupled from transcription regulation in the protein-coding direction. We further provide evidence that Rap1 acts independently of chromatin-based mechanisms to repress cryptic or divergent transcription. Finally, we show that divergent transcription in the absence of Rap1 is elicited by the RSC chromatin remodeller. We propose that a sequence-specific transcription factor limits access of basal transcription machinery to regulatory elements and adjacent sequences that act as divergent cryptic promoters, thereby providing directionality towards productive transcription..
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Preprint| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
bioRxiv.org - (2022) vom: 23. Aug. Zur Gesamtaufnahme - year:2022 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wu, Andrew CK [VerfasserIn] |
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| doi: |
10.1101/314310 |
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| PPN (Katalog-ID): |
XBI036970972 |
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| 100 | 1 | |a Wu, Andrew CK |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Repression of divergent noncoding transcription by a sequence-specific transcription factor |
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| 520 | |a Summary Many active eukaryotic gene promoters exhibit divergent noncoding transcription, but the mechanisms restricting expression of these transcripts are not well understood. Here we demonstrate how a sequence-specific transcription factor represses divergent noncoding transcription at highly expressed genes in yeast. We find that depletion of the transcription factor Rap1 induces noncoding transcription in a large fraction of Rap1 regulated gene promoters. Specifically, Rap1 prevents transcription initiation at cryptic promoters near its binding sites, which is uncoupled from transcription regulation in the protein-coding direction. We further provide evidence that Rap1 acts independently of chromatin-based mechanisms to repress cryptic or divergent transcription. Finally, we show that divergent transcription in the absence of Rap1 is elicited by the RSC chromatin remodeller. We propose that a sequence-specific transcription factor limits access of basal transcription machinery to regulatory elements and adjacent sequences that act as divergent cryptic promoters, thereby providing directionality towards productive transcription. | ||
| 650 | 4 | |a Biology |7 (dpeaa)DE-84 | |
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| 700 | 1 | |a Patel, Harshil |e verfasserin |4 aut | |
| 700 | 1 | |a Chia, Minghao |e verfasserin |4 aut | |
| 700 | 1 | |a Moretto, Fabien |e verfasserin |4 aut | |
| 700 | 1 | |a Frith, David |e verfasserin |4 aut | |
| 700 | 1 | |a Snijders, Ambrosius P. |e verfasserin |4 aut | |
| 700 | 1 | |a van Werven, Folkert |e verfasserin |4 aut | |
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