<i>Legionella pneumophila</i>occurrence in reduced-occupancy buildings in 11 cities during the COVID-19 pandemic
Abstract In spring 2020, reduced water demand was an unintended consequence of COVID-19 pandemic-related building closures. Concerns arose that contaminants associated with water stagnation, such asLegionella pneumophila, could become prevalent. To investigate this potential public health risk, samples from 26 reduced-occupancy buildings across 11 cities in the United States, Canada, and Switzerland were analyzed forL. pneumophilausing liquid culture (Legiolert, n=258) and DNA-based methods (qPCR/ddPCR, n=138).L. pneumophilaculture-positivity was largely associated with just five buildings, each of which had specific design or operational deficiencies commonly associated withL. pneumophilaoccurrence. Samples from free chlorine buildings had higher culture-positivity (37%) than chloramine buildings (1%), and 78% of culture-positive samples occurred when the residual was ≤0.1 mg/L Cl2. Although overall sample positivities using culture- and DNA-based methods were equivalent (34% vs. 35%), there was disagreement between the methods in 13% of paired samples. Few buildings reported any water management activities, andL. pneumophilaconcentrations in flushed samples were occasionally greater than in first-draw samples. This study provides insight into how building plumbing characteristics and management practices contribute toL. pneumophilaoccurrence during low water use periods and can inform targeted prevention and mitigation efforts.Synopsis Statement Legionella pneumophilaoccurrence was evaluated in reduced-occupancy buildings during the COVID-19 pandemic across multiple cities.Graphic for Table of Contents (TOC) <jats:fig id="ufig1" position="float" fig-type="figure" orientation="portrait"><jats:graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="22277022v1_ufig1" position="float" orientation="portrait" /></jats:fig>.
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Preprint| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
bioRxiv.org - (2024) vom: 23. Apr. Zur Gesamtaufnahme - year:2024 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Dowdell, Katherine S. [VerfasserIn] |
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| doi: |
10.1101/2022.06.28.22277022 |
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| PPN (Katalog-ID): |
XBI03640439X |
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| 100 | 1 | |a Dowdell, Katherine S. |e verfasserin |0 (orcid)0000-0001-5812-9577 |4 aut | |
| 245 | 1 | 0 | |a <i>Legionella pneumophila</i>occurrence in reduced-occupancy buildings in 11 cities during the COVID-19 pandemic |
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| 520 | |a Abstract In spring 2020, reduced water demand was an unintended consequence of COVID-19 pandemic-related building closures. Concerns arose that contaminants associated with water stagnation, such asLegionella pneumophila, could become prevalent. To investigate this potential public health risk, samples from 26 reduced-occupancy buildings across 11 cities in the United States, Canada, and Switzerland were analyzed forL. pneumophilausing liquid culture (Legiolert, n=258) and DNA-based methods (qPCR/ddPCR, n=138).L. pneumophilaculture-positivity was largely associated with just five buildings, each of which had specific design or operational deficiencies commonly associated withL. pneumophilaoccurrence. Samples from free chlorine buildings had higher culture-positivity (37%) than chloramine buildings (1%), and 78% of culture-positive samples occurred when the residual was ≤0.1 mg/L Cl2. Although overall sample positivities using culture- and DNA-based methods were equivalent (34% vs. 35%), there was disagreement between the methods in 13% of paired samples. Few buildings reported any water management activities, andL. pneumophilaconcentrations in flushed samples were occasionally greater than in first-draw samples. This study provides insight into how building plumbing characteristics and management practices contribute toL. pneumophilaoccurrence during low water use periods and can inform targeted prevention and mitigation efforts.Synopsis Statement Legionella pneumophilaoccurrence was evaluated in reduced-occupancy buildings during the COVID-19 pandemic across multiple cities.Graphic for Table of Contents (TOC) <jats:fig id="ufig1" position="float" fig-type="figure" orientation="portrait"><jats:graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="22277022v1_ufig1" position="float" orientation="portrait" /></jats:fig> | ||
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| 700 | 1 | |a Grimard-Conea, Marianne |e verfasserin |4 aut | |
| 700 | 1 | |a Pitell, Sarah |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Yang |e verfasserin |4 aut | |
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