A global lipid map reveals host dependency factors conserved across SARS-CoV-2 variants
Abstract A comprehensive understanding of host dependency factors for SARS-CoV-2 remains elusive. We mapped alterations in host lipids following SARS-CoV-2 infection using nontargeted lipidomics. We found that SARS-CoV-2 rewires host lipid metabolism, altering 409 lipid species up to 64-fold relative to controls. We correlated these changes with viral protein activity by transfecting human cells with each viral protein and performing lipidomics. We found that lipid droplet plasticity is a key feature of infection and that viral propagation can be blocked by small-molecule glycerolipid biosynthesis inhibitors. We found that this inhibition was effective against the main variants of concern (alpha, beta, gamma, and delta), indicating that glycerolipid biosynthesis is a conserved host dependency factor that supports this evolving virus..
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Preprint| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
bioRxiv.org - (2023) vom: 17. Nov. Zur Gesamtaufnahme - year:2023 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Farley, Scotland E. [VerfasserIn] |
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| doi: |
10.1101/2022.02.14.480430 |
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| PPN (Katalog-ID): |
XBI03525789X |
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| 245 | 1 | 0 | |a A global lipid map reveals host dependency factors conserved across SARS-CoV-2 variants |
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| 520 | |a Abstract A comprehensive understanding of host dependency factors for SARS-CoV-2 remains elusive. We mapped alterations in host lipids following SARS-CoV-2 infection using nontargeted lipidomics. We found that SARS-CoV-2 rewires host lipid metabolism, altering 409 lipid species up to 64-fold relative to controls. We correlated these changes with viral protein activity by transfecting human cells with each viral protein and performing lipidomics. We found that lipid droplet plasticity is a key feature of infection and that viral propagation can be blocked by small-molecule glycerolipid biosynthesis inhibitors. We found that this inhibition was effective against the main variants of concern (alpha, beta, gamma, and delta), indicating that glycerolipid biosynthesis is a conserved host dependency factor that supports this evolving virus. | ||
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| 700 | 1 | |a Kyle, Jennifer E. |4 aut | |
| 700 | 1 | |a Leier, Hans C. |4 aut | |
| 700 | 1 | |a Bramer, Lisa M. |4 aut | |
| 700 | 1 | |a Weinstein, Jules |4 aut | |
| 700 | 1 | |a Bates, Timothy A. |4 aut | |
| 700 | 1 | |a Lee, Joon-Yong |4 aut | |
| 700 | 1 | |a Metz, Thomas O. |4 aut | |
| 700 | 1 | |a Schultz, Carsten |4 aut | |
| 700 | 1 | |a Tafesse, Fikadu G. |0 (orcid)0000-0002-8575-4164 |4 aut | |
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