Deciphering the quality of SARS-CoV-2 specific T-cell response associated with disease severity, immune memory and heterologous response
ABSTRACT SARS-CoV-2 specific T-cell response has been associated with disease severity, immune memory and heterologous response to endemic coronaviruses. However, an integrative approach combining a comprehensive analysis of the quality of SARS-CoV-2 specific T-cell response with antibody levels in these three scenarios is needed. In the present study we found that, in acute infection, while mild disease was associated with high T-cell polyfunctionality biased to IL-2 production and inversely correlated with anti-S IgG levels, combinations only including IFN-γ with absence of perforin production predominated in severe disease. Seven months after infection, both non-hospitalized and previously hospitalized patients presented robust anti-S IgG levels and SARS-CoV-2 specific T-cell response. In addition, only previously hospitalized patients showed a T-cell exhaustion profile. Finally, combinations including IL-2 in response to S protein of endemic coronaviruses, were the ones associated with SARS-CoV-2 S-specific T-cell response in pre-COVID-19 healthy donors’ samples. These results have implications for protective immunity against SARS-CoV-2 and recurrent COVID-19 and may help for the design of new prototypes and boosting vaccine strategies..
| Medienart: |
|
|---|
Preprint| Erscheinungsjahr: |
2022 |
|---|---|
| Erschienen: |
2022 |
| Enthalten in: |
bioRxiv.org - (2022) vom: 25. Mai Zur Gesamtaufnahme - year:2022 |
|---|
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Pérez-Gómez, Alberto [VerfasserIn] |
|---|
| Links: |
|---|
| doi: |
10.1101/2021.12.28.474325 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
XBI033309795 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | XBI033309795 | ||
| 003 | DE-627 | ||
| 005 | 20230429100955.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 211230s2022 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1101/2021.12.28.474325 |2 doi | |
| 035 | |a (DE-627)XBI033309795 | ||
| 035 | |a (biorXiv)10.1101/2021.12.28.474325 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | |a 570 |q DE-84 | |
| 100 | 1 | |a Pérez-Gómez, Alberto |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Deciphering the quality of SARS-CoV-2 specific T-cell response associated with disease severity, immune memory and heterologous response |
| 264 | 1 | |c 2022 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a ABSTRACT SARS-CoV-2 specific T-cell response has been associated with disease severity, immune memory and heterologous response to endemic coronaviruses. However, an integrative approach combining a comprehensive analysis of the quality of SARS-CoV-2 specific T-cell response with antibody levels in these three scenarios is needed. In the present study we found that, in acute infection, while mild disease was associated with high T-cell polyfunctionality biased to IL-2 production and inversely correlated with anti-S IgG levels, combinations only including IFN-γ with absence of perforin production predominated in severe disease. Seven months after infection, both non-hospitalized and previously hospitalized patients presented robust anti-S IgG levels and SARS-CoV-2 specific T-cell response. In addition, only previously hospitalized patients showed a T-cell exhaustion profile. Finally, combinations including IL-2 in response to S protein of endemic coronaviruses, were the ones associated with SARS-CoV-2 S-specific T-cell response in pre-COVID-19 healthy donors’ samples. These results have implications for protective immunity against SARS-CoV-2 and recurrent COVID-19 and may help for the design of new prototypes and boosting vaccine strategies. | ||
| 700 | 1 | |a Gasca-Capote, M Carmen |e verfasserin |4 aut | |
| 700 | 1 | |a Vitallé, Joana |e verfasserin |4 aut | |
| 700 | 1 | |a Ostos, Francisco J. |e verfasserin |4 aut | |
| 700 | 1 | |a Serna-Gallego, Ana |e verfasserin |4 aut | |
| 700 | 1 | |a Trujillo-Rodríguez, María |e verfasserin |4 aut | |
| 700 | 1 | |a Muñoz-Muela, Esperanza |e verfasserin |4 aut | |
| 700 | 1 | |a Giráldez-Pérez, Teresa |e verfasserin |4 aut | |
| 700 | 1 | |a Praena-Segovia, Julia |e verfasserin |4 aut | |
| 700 | 1 | |a Navarro-Amuedo, María D. |e verfasserin |4 aut | |
| 700 | 1 | |a Paniagua-García, María |e verfasserin |4 aut | |
| 700 | 1 | |a García-Gutiérrez, Manuel |e verfasserin |4 aut | |
| 700 | 1 | |a Aguilar-Guisado, Manuela |e verfasserin |4 aut | |
| 700 | 1 | |a Rivas-Jeremias, Inmaculada |e verfasserin |4 aut | |
| 700 | 1 | |a Jimenez-Leon, María R. |e verfasserin |4 aut | |
| 700 | 1 | |a Bachiller, Sara |e verfasserin |4 aut | |
| 700 | 1 | |a Fernández-Villar, Alberto |e verfasserin |4 aut | |
| 700 | 1 | |a Pérez-González, Alexandre |e verfasserin |4 aut | |
| 700 | 1 | |a Gutiérrez-Valencia, Alicia |e verfasserin |4 aut | |
| 700 | 1 | |a Benhnia, Mohammed Rafii-El-Idrissi |e verfasserin |4 aut | |
| 700 | 1 | |a Weiskopf, Daniela |e verfasserin |4 aut | |
| 700 | 1 | |a Sette, Alessandro |e verfasserin |4 aut | |
| 700 | 1 | |a López-Cortes, Luis F. |e verfasserin |4 aut | |
| 700 | 1 | |a Poveda, Eva |e verfasserin |4 aut | |
| 700 | 1 | |a Ruiz-Mateos, Ezequiel |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t bioRxiv.org |g (2022) vom: 25. Mai |
| 773 | 1 | 8 | |g year:2022 |g day:25 |g month:05 |
| 856 | 4 | 0 | |u https://doi.org/10.1002/ctm2.802 |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1101/2021.12.28.474325 |z kostenfrei |3 Volltext |
| 912 | |a GBV_XBI | ||
| 912 | |a SSG-OLC-PHA | ||
| 951 | |a AR | ||
| 952 | |j 2022 |b 25 |c 05 | ||
| 953 | |2 045F |a 570 | ||