Zooplankton as a transitional host for<i>Escherichia coli</i>in freshwater
Abstract This study shows thatEscherichia colican be temporarily enriched in zooplankton in natural conditions and that these bacteria can belong to different phylogroups and sequence types including environmental as well as clinical and animal isolates. We isolated 10E. colistrains and sequenced the genomes of two of them. Phylogenetically the two isolates were closer to strains isolated from poultry meat than with freshwaterE. coli, albeit their genomes were smaller than those from poultry. After isolation and fluorescent protein tagging of strains ED1 and ED157 we show thatDaphniasp. can take up these strains and release them alive again, thus forming a temporary host forE. coli. In a chemostat experiment we show that the association does not prolong the bacterial long-term survival, but that at low abundances it does also not significantly reduce the bacterial numbers. We demonstrate thatE. colidoes not belong to the core microbiota ofDaphnia, suffers from competition by the natural microbiota ofDaphnia, but can profit from its carapax to survive in water. All in all, this study suggests that the association ofE. colitoDaphniais only temporary but that the cells are viable therein and this might allow encounters with other bacteria for genetic exchange and potential genomic adaptations to the freshwater environment.Importance The contamination of freshwaters with faecal derived bacteria is of major concern regarding drinking water acquisition and recreational activities. Ecological interactions promoting their persistence are still very scarcely studied. This study, which analyses the survival ofE. coliin the presence of zooplankton, is thus of ecological as well as water safety relevance..
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Preprint| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
bioRxiv.org - (2023) vom: 14. Nov. Zur Gesamtaufnahme - year:2023 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
di Cesare, Andrea [VerfasserIn] |
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| doi: |
10.1101/2021.12.23.474077 |
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| PPN (Katalog-ID): |
XBI033298173 |
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| 520 | |a Abstract This study shows thatEscherichia colican be temporarily enriched in zooplankton in natural conditions and that these bacteria can belong to different phylogroups and sequence types including environmental as well as clinical and animal isolates. We isolated 10E. colistrains and sequenced the genomes of two of them. Phylogenetically the two isolates were closer to strains isolated from poultry meat than with freshwaterE. coli, albeit their genomes were smaller than those from poultry. After isolation and fluorescent protein tagging of strains ED1 and ED157 we show thatDaphniasp. can take up these strains and release them alive again, thus forming a temporary host forE. coli. In a chemostat experiment we show that the association does not prolong the bacterial long-term survival, but that at low abundances it does also not significantly reduce the bacterial numbers. We demonstrate thatE. colidoes not belong to the core microbiota ofDaphnia, suffers from competition by the natural microbiota ofDaphnia, but can profit from its carapax to survive in water. All in all, this study suggests that the association ofE. colitoDaphniais only temporary but that the cells are viable therein and this might allow encounters with other bacteria for genetic exchange and potential genomic adaptations to the freshwater environment.Importance The contamination of freshwaters with faecal derived bacteria is of major concern regarding drinking water acquisition and recreational activities. Ecological interactions promoting their persistence are still very scarcely studied. This study, which analyses the survival ofE. coliin the presence of zooplankton, is thus of ecological as well as water safety relevance. | ||
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| 700 | 1 | |a Colinas, Noemi |4 aut | |
| 700 | 1 | |a Borgomaneiro, Giulia |4 aut | |
| 700 | 1 | |a Borin, Sara |4 aut | |
| 700 | 1 | |a Cabello-Yeves, Pedro J. |4 aut | |
| 700 | 1 | |a Canale, Claudia |4 aut | |
| 700 | 1 | |a Cedraro, Nicholas |4 aut | |
| 700 | 1 | |a Citterio, Barbara |4 aut | |
| 700 | 1 | |a Crotti, Elena |4 aut | |
| 700 | 1 | |a Mangiaterra, Gianmarco |4 aut | |
| 700 | 1 | |a Mapelli, Francesca |4 aut | |
| 700 | 1 | |a Mondino, Vincenzo |4 aut | |
| 700 | 1 | |a Vignaroli, Carla |0 (orcid)0000-0002-0993-5967 |4 aut | |
| 700 | 1 | |a Quaranta, Walter |4 aut | |
| 700 | 1 | |a Corno, Gianluca |4 aut | |
| 700 | 1 | |a Fontaneto, Diego |4 aut | |
| 700 | 1 | |a Eckert, Ester M |0 (orcid)0000-0002-7314-1715 |4 aut | |
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