Genotype and Th2 cells control monocyte to tissue resident macrophage differentiation during nematode infection of the pleural cavity
Abstract The recent revolution in tissue-resident macrophage biology has resulted largely from murine studies performed in the C57BL/6 strain. Here, we provide a comprehensive analysis of immune cells in the pleural cavity using both C57BL/6 and BALB/c mice. Unlike C57BL/6 mice, naïve tissue-resident Large Cavity Macrophages (LCM) of BALB/c mice failed to fully implement the tissue residency program. Following infection with a pleural-dwelling nematode these pre-existing differences were accentuated with LCM expansion occurring in C57BL/6 but not BALB/c mice. While infection drove monocyte recruitment in both strains, only in C57BL/6 mice were monocytes able to efficiently integrate into the resident pool. Monocyte to macrophage conversion required both T cells and IL-4Rα signalling. Host genetics are therefore a key influence on tissue resident macrophage biology, and during nematode infection Th2 cells control the differentiation pathway of tissue resident macrophages.Graphical Abstract <jats:fig id="ufig1" position="float" fig-type="figure" orientation="portrait"><jats:graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="472661v1_ufig1" position="float" orientation="portrait" /></jats:fig>.
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Preprint| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
bioRxiv.org - (2023) vom: 14. Nov. Zur Gesamtaufnahme - year:2023 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Finlay, Conor M [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.1101/2021.12.17.472661 |
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| PPN (Katalog-ID): |
XBI033256349 |
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| 245 | 1 | 0 | |a Genotype and Th2 cells control monocyte to tissue resident macrophage differentiation during nematode infection of the pleural cavity |
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| 520 | |a Abstract The recent revolution in tissue-resident macrophage biology has resulted largely from murine studies performed in the C57BL/6 strain. Here, we provide a comprehensive analysis of immune cells in the pleural cavity using both C57BL/6 and BALB/c mice. Unlike C57BL/6 mice, naïve tissue-resident Large Cavity Macrophages (LCM) of BALB/c mice failed to fully implement the tissue residency program. Following infection with a pleural-dwelling nematode these pre-existing differences were accentuated with LCM expansion occurring in C57BL/6 but not BALB/c mice. While infection drove monocyte recruitment in both strains, only in C57BL/6 mice were monocytes able to efficiently integrate into the resident pool. Monocyte to macrophage conversion required both T cells and IL-4Rα signalling. Host genetics are therefore a key influence on tissue resident macrophage biology, and during nematode infection Th2 cells control the differentiation pathway of tissue resident macrophages.Graphical Abstract <jats:fig id="ufig1" position="float" fig-type="figure" orientation="portrait"><jats:graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="472661v1_ufig1" position="float" orientation="portrait" /></jats:fig> | ||
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| 700 | 1 | |a Chan, Brian HK |4 aut | |
| 700 | 1 | |a Ajendra, Jesuthas |0 (orcid)0000-0002-9256-5396 |4 aut | |
| 700 | 1 | |a Chenery, Alistair |0 (orcid)0000-0001-8755-461X |4 aut | |
| 700 | 1 | |a Morrison, Anya |4 aut | |
| 700 | 1 | |a Houlder, Emma L |0 (orcid)0000-0003-0448-6055 |4 aut | |
| 700 | 1 | |a Baker, Syed Murtuza |0 (orcid)0000-0002-6633-333X |4 aut | |
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| 700 | 1 | |a Allen, Judith E |0 (orcid)0000-0002-3829-066X |4 aut | |
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