Mucosal antibody response to SARS-CoV-2 in paediatric and adult patients: a longitudinal study
Abstract Conjunctival and nasal mucosal antibody responses in thirty-four paediatric and forty-seven adult COVID-19 patients were measured. The mucosal antibody was IgA dominant. In the nasal epithelial lining fluid (NELF) of asymptomatic paediatric patients, SARS-CoV-2 spike protein 1 (S1) specific immunoglobulin A (IgA) was induced early. Their plasma S1-specific IgG levels were higher than symptomatic patients. More adult with mild disease had NELF S1-specific IgA than those with severe/critical illness. Within the first week of diagnosis, higher S1-specific antibodies in NELF and plasma and lower vial loads were detected in paediatric than adult patients with mild disease. The IgA and IgG levels correlated positively with the surrogate neutralization readout. The detectable NELF ‘neutralizing’ S1-specific IgA in the first week after diagnosis correlated with a rapid decline in viral load. This study highlights the effect of nasal IgA in limiting the SARS-CoV-2 replication and provides complementary information to the serum antibody measurements..
| Medienart: |
|
|---|
Preprint| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
bioRxiv.org - (2023) vom: 10. Jan. Zur Gesamtaufnahme - year:2023 |
|---|
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Chan, Renee WY [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
|---|
| doi: |
10.1101/2021.09.27.21264219 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
XBI032683804 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | XBI032683804 | ||
| 003 | DE-627 | ||
| 005 | 20230429082635.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 210930s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1101/2021.09.27.21264219 |2 doi | |
| 035 | |a (DE-627)XBI032683804 | ||
| 035 | |a (biorXiv)10.1101/2021.09.27.21264219 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Chan, Renee WY |e verfasserin |0 (orcid)0000-0002-0682-9587 |4 aut | |
| 245 | 1 | 0 | |a Mucosal antibody response to SARS-CoV-2 in paediatric and adult patients: a longitudinal study |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a Abstract Conjunctival and nasal mucosal antibody responses in thirty-four paediatric and forty-seven adult COVID-19 patients were measured. The mucosal antibody was IgA dominant. In the nasal epithelial lining fluid (NELF) of asymptomatic paediatric patients, SARS-CoV-2 spike protein 1 (S1) specific immunoglobulin A (IgA) was induced early. Their plasma S1-specific IgG levels were higher than symptomatic patients. More adult with mild disease had NELF S1-specific IgA than those with severe/critical illness. Within the first week of diagnosis, higher S1-specific antibodies in NELF and plasma and lower vial loads were detected in paediatric than adult patients with mild disease. The IgA and IgG levels correlated positively with the surrogate neutralization readout. The detectable NELF ‘neutralizing’ S1-specific IgA in the first week after diagnosis correlated with a rapid decline in viral load. This study highlights the effect of nasal IgA in limiting the SARS-CoV-2 replication and provides complementary information to the serum antibody measurements. | ||
| 650 | 4 | |a Biology |7 (dpeaa)DE-84 | |
| 650 | 4 | |a 570 |7 (dpeaa)DE-84 | |
| 700 | 1 | |a Chan, Kate CC |4 aut | |
| 700 | 1 | |a Lui, Grace CY |4 aut | |
| 700 | 1 | |a Tsun, Joseph GS |4 aut | |
| 700 | 1 | |a Chan, Kathy YY |4 aut | |
| 700 | 1 | |a Yip, Jasmine SK |4 aut | |
| 700 | 1 | |a Liu, Shaojun |4 aut | |
| 700 | 1 | |a Yu, Michelle WL |4 aut | |
| 700 | 1 | |a Ng, Rita WY |4 aut | |
| 700 | 1 | |a Chong, Kelvin KL |4 aut | |
| 700 | 1 | |a Wang, Maggie H |0 (orcid)0000-0001-6485-0926 |4 aut | |
| 700 | 1 | |a Chan, Paul KS |4 aut | |
| 700 | 1 | |a Li, Albert M |4 aut | |
| 700 | 1 | |a Lam, Hugh S |0 (orcid)0000-0001-9912-0544 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t bioRxiv.org |g (2023) vom: 10. Jan. |
| 773 | 1 | 8 | |g year:2023 |g day:10 |g month:01 |
| 856 | 4 | 0 | |u https://doi.org/10.3390/pathogens11040397 |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1101/2021.09.27.21264219 |z kostenfrei |3 Volltext |
| 912 | |a GBV_XBI | ||
| 912 | |a SSG-OLC-PHA | ||
| 951 | |a AR | ||
| 952 | |j 2023 |b 10 |c 01 | ||