Defective<i>Slc7a7</i>transport reduces erythropoietin compromising erythropoiesis and iron homeostasis

Abstract Lysinuric Protein Intolerance (LPI) is an inborn error of metabolism resulting fromSLC7A7deficiency that causes diminished plasma concentration of cationic amino acids. The clinical picture is highly heterogeneous among patients, who commonly present intolerance to protein intake and more severe complications such as hematological abnormalities and kidney failure. Although current treatments aim to address the metabolic defects of LPI, they have been unsatisfactory when treating the most severe symptoms. Here we show that the absence ofSlc7a7in mice causes iron overload as a result of erythropoiesis failure. Regarding iron metabolism, we demonstrate that reduced plasma erythropoietin triggers a strong iron overload, as erythropoietin administration restores normal iron levels and mitigate hematological alterations. Interestingly, we found that human LPI is associated with hyperferritinemia but not iron overload, a trait that might be influenced by the citrulline treatment. Furthermore, we show that erythropoietin is a key factor in the hematological abnormalities in LPI. Our study reveals a mechanism leading to LPI-induced hematological complications and identifies erythropoietin supplementation as a promising therapeutic strategy for human LPI.Significance Statement The systemic metabolic environment derived fromSlc7a7-ablation in epithelial cells from kidney and intestine causes erythropoiesis failure prompting therefore iron overload. Here, we identify erythropoietin as the main driver of erythropoiesis failure as exogenous erythropoietin administration restores normal erythroblast population. In addition, we have also analyzed human data and found that patients with LPI have abnormal ferritin levels. Finally, as human LPI, citrulline treatment in mice restores normal iron homeostasis, highlighting the relevance of the systemic environment in LPI. Erythropoietin supplementation emerges as a promising therapeutic strategy for human LPI without the inflammatory effect associated with citrulline supplementation..

Medienart:	Preprint

Erscheinungsjahr:	2023
Erschienen:	2023

Enthalten in:	bioRxiv.org - (2023) vom: 07. Nov. Zur Gesamtaufnahme - year:2023

Sprache:	Englisch

Beteiligte Personen:

Giroud-Gerbetant, Judith [VerfasserIn] Sotillo, Fernando [VerfasserIn] Hernández, Gonzalo [VerfasserIn] Lynch, Cian J [VerfasserIn] Ruano, Irene [VerfasserIn] Siri, Barbara [VerfasserIn] Sebastian, David [VerfasserIn] Zorzano, Antonio [VerfasserIn] Artuch, Rafael [VerfasserIn] Ormazabal, Aida [VerfasserIn] Sánchez, Mayka [VerfasserIn] Weiss, Günter [VerfasserIn] Prats, Neus [VerfasserIn] Dionisi-Vici, Carlo [VerfasserIn] Serrano, Manuel [VerfasserIn] Palacín, Manuel [VerfasserIn] Bodoy, Susanna [VerfasserIn]

Links:	Volltext [kostenfrei]

Themen:	570 Biology

doi:	10.1101/2021.08.15.456393

funding:
Förderinstitution / Projekttitel:

PPN (Katalog-ID):	XBI032394888