Combinatorial drug-microenvironment interaction mapping reveals cell-extrinsic drug resistance mechanisms and clinically relevant patient subgroups in CLL
Abstract The tumour microenvironment and genetic alterations collectively influence drug efficacy in cancer, but current evidence is limited to small scale studies and systematic analyses are lacking. We chose Chronic Lymphocytic Leukaemia (CLL), the most common leukaemia in adults, as a model disease to study this complex interplay systematically. We performed a combinatorial assay using 12 drugs individually co-applied with each of 17 microenvironmental stimuli in 192 primary CLL samples, generating a comprehensive map of drug-microenvironment interactions in CLL. This data was combined with whole-exome sequencing, DNA-methylation, RNA-sequencing and copy number variant annotation. Our assay identified four distinct CLL subgroups that differed in their responses to the panel of microenvironmental stimuli. These subgroups were characterized by distinct clinical outcomes independently of known prognostic markers. We investigated the effect of CLL- specific recurrent genetic alterations on microenvironmental responses and identified trisomy 12 as an amplifier of multiple microenvironmental stimuli. We further quantified the impact of microenvironmental stimuli on drug response, confirmed known interactions such as Interleukin (IL) 4 mediated resistance to B cell receptor (BCR) inhibitors, and identified new interactions such as Interferon-γ induced resistance to BCR inhibitors. Finally, we identified interactions which were limited to genetic subgroups. Resistance to chemotherapeutics, such as Fludarabine, induced by Toll-Like Receptor (TLR) agonists could be observed in IGHV unmutated patient samples and IGHV mutated samples with trisomy 12. In-vivo relevance was investigated in CLL-infiltrated lymph nodes, which showed increased IL4 and TLR signalling activity compared to healthy samples (p<0.001). High IL4 activity in lymph nodes correlated with faster disease progression (p=0.038).We provide a publicly available resource (<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://www.dietrichlab.de/CLL_Microenvironment/">www.dietrichlab.de/CLL_Microenvironment/</jats:ext-link>) which uncovers tumour cell extrinsic influences on drug response and disease progression in CLL, and how these interactions are modulated by cell intrinsic molecular features..
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Preprint| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
bioRxiv.org - (2023) vom: 06. Nov. Zur Gesamtaufnahme - year:2023 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Bruch, Peter-Martin [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.1101/2021.07.23.453514 |
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| PPN (Katalog-ID): |
XBI032250053 |
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| 100 | 1 | |a Bruch, Peter-Martin |e verfasserin |0 (orcid)0000-0002-9992-3109 |4 aut | |
| 245 | 1 | 0 | |a Combinatorial drug-microenvironment interaction mapping reveals cell-extrinsic drug resistance mechanisms and clinically relevant patient subgroups in CLL |
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| 520 | |a Abstract The tumour microenvironment and genetic alterations collectively influence drug efficacy in cancer, but current evidence is limited to small scale studies and systematic analyses are lacking. We chose Chronic Lymphocytic Leukaemia (CLL), the most common leukaemia in adults, as a model disease to study this complex interplay systematically. We performed a combinatorial assay using 12 drugs individually co-applied with each of 17 microenvironmental stimuli in 192 primary CLL samples, generating a comprehensive map of drug-microenvironment interactions in CLL. This data was combined with whole-exome sequencing, DNA-methylation, RNA-sequencing and copy number variant annotation. Our assay identified four distinct CLL subgroups that differed in their responses to the panel of microenvironmental stimuli. These subgroups were characterized by distinct clinical outcomes independently of known prognostic markers. We investigated the effect of CLL- specific recurrent genetic alterations on microenvironmental responses and identified trisomy 12 as an amplifier of multiple microenvironmental stimuli. We further quantified the impact of microenvironmental stimuli on drug response, confirmed known interactions such as Interleukin (IL) 4 mediated resistance to B cell receptor (BCR) inhibitors, and identified new interactions such as Interferon-γ induced resistance to BCR inhibitors. Finally, we identified interactions which were limited to genetic subgroups. Resistance to chemotherapeutics, such as Fludarabine, induced by Toll-Like Receptor (TLR) agonists could be observed in IGHV unmutated patient samples and IGHV mutated samples with trisomy 12. In-vivo relevance was investigated in CLL-infiltrated lymph nodes, which showed increased IL4 and TLR signalling activity compared to healthy samples (p<0.001). High IL4 activity in lymph nodes correlated with faster disease progression (p=0.038).We provide a publicly available resource (<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://www.dietrichlab.de/CLL_Microenvironment/">www.dietrichlab.de/CLL_Microenvironment/</jats:ext-link>) which uncovers tumour cell extrinsic influences on drug response and disease progression in CLL, and how these interactions are modulated by cell intrinsic molecular features. | ||
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| 700 | 1 | |a Giles, Holly A. R. |0 (orcid)0000-0002-9295-587X |4 aut | |
| 700 | 1 | |a Kolb, Carolin |4 aut | |
| 700 | 1 | |a Herbst, Sophie A. |0 (orcid)0000-0001-8502-0366 |4 aut | |
| 700 | 1 | |a Becirovic, Tina |4 aut | |
| 700 | 1 | |a Roider, Tobias |0 (orcid)0000-0002-6973-3531 |4 aut | |
| 700 | 1 | |a Lu, Junyan |0 (orcid)0000-0002-9211-0746 |4 aut | |
| 700 | 1 | |a Scheinost, Sebastian |4 aut | |
| 700 | 1 | |a Wagner, Lena |4 aut | |
| 700 | 1 | |a Huellein, Jennifer |4 aut | |
| 700 | 1 | |a Berest, Ivan |0 (orcid)0000-0001-7607-9163 |4 aut | |
| 700 | 1 | |a Kriegsmann, Mark |4 aut | |
| 700 | 1 | |a Kriegsmann, Katharina |0 (orcid)0000-0002-3620-1491 |4 aut | |
| 700 | 1 | |a Zgorzelski, Christiane |4 aut | |
| 700 | 1 | |a Dreger, Peter |0 (orcid)0000-0002-7429-8570 |4 aut | |
| 700 | 1 | |a Zaugg, Judith B. |0 (orcid)0000-0001-8324-4040 |4 aut | |
| 700 | 1 | |a Müller-Tidow, Carsten |4 aut | |
| 700 | 1 | |a Zenz, Thorsten |4 aut | |
| 700 | 1 | |a Huber, Wolfgang |0 (orcid)0000-0002-0474-2218 |4 aut | |
| 700 | 1 | |a Dietrich, Sascha |0 (orcid)0000-0002-0648-1832 |4 aut | |
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