Human organoid systems reveal in vitro correlates of fitness for SARS-CoV-2 B.1.1.7
Abstract A new phase of the COVID-19 pandemic has started as several SARS-CoV-2 variants are rapidly emerging globally, raising concerns for increased transmissibility. As animal models and traditional in vitro systems may fail to model key aspects of the SARS-CoV-2 replication cycle, representative in vitro systems to assess variants phenotypically are urgently needed. We found that the British variant (clade B.1.1.7), compared to an ancestral SARS-CoV-2 clade B virus, produced higher levels of infectious virus late in infection and had a higher replicative fitness in human airway, alveolar and intestinal organoid models. Our findings unveil human organoids as powerful tools to phenotype viral variants and suggest extended shedding as a correlate of fitness for SARS-CoV-2.One-Sentence Summary British SARS-CoV-2 variant (clade B.1.1.7) infects organoids for extended time and has a higher fitness in vitro..
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Preprint| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
bioRxiv.org - (2021) vom: 05. Mai Zur Gesamtaufnahme - year:2021 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Lamers, Mart M. [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.1101/2021.05.03.441080 |
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| PPN (Katalog-ID): |
XBI020486804 |
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| 520 | |a Abstract A new phase of the COVID-19 pandemic has started as several SARS-CoV-2 variants are rapidly emerging globally, raising concerns for increased transmissibility. As animal models and traditional in vitro systems may fail to model key aspects of the SARS-CoV-2 replication cycle, representative in vitro systems to assess variants phenotypically are urgently needed. We found that the British variant (clade B.1.1.7), compared to an ancestral SARS-CoV-2 clade B virus, produced higher levels of infectious virus late in infection and had a higher replicative fitness in human airway, alveolar and intestinal organoid models. Our findings unveil human organoids as powerful tools to phenotype viral variants and suggest extended shedding as a correlate of fitness for SARS-CoV-2.One-Sentence Summary British SARS-CoV-2 variant (clade B.1.1.7) infects organoids for extended time and has a higher fitness in vitro. | ||
| 700 | 1 | |a Breugem, Tim I. |e verfasserin |4 aut | |
| 700 | 1 | |a Mykytyn, Anna Z. |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Yiquan |e verfasserin |4 aut | |
| 700 | 1 | |a Groen, Nathalie |e verfasserin |4 aut | |
| 700 | 1 | |a Knoops, Kèvin |e verfasserin |4 aut | |
| 700 | 1 | |a Schipper, Debby |e verfasserin |4 aut | |
| 700 | 1 | |a van der Vaart, Jelte |e verfasserin |4 aut | |
| 700 | 1 | |a Koopman, Charlotte D. |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Jingshu |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Douglas C. |e verfasserin |4 aut | |
| 700 | 1 | |a van den Doel, Petra B. |e verfasserin |4 aut | |
| 700 | 1 | |a Bestebroer, Theo |e verfasserin |4 aut | |
| 700 | 1 | |a GeurtsvanKessel, Corine H. |e verfasserin |4 aut | |
| 700 | 1 | |a Peters, Peter J. |e verfasserin |4 aut | |
| 700 | 1 | |a Muraro, Mauro J. |e verfasserin |4 aut | |
| 700 | 1 | |a Clevers, Hans |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Nicholas C. |e verfasserin |4 aut | |
| 700 | 1 | |a Haagmans, Bart L. |e verfasserin |4 aut | |
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