Identification of unknown proteins in X-ray crystallography and cryo-EM
Abstract Although experimental protein structure determination usually targets known proteins, chains of unknown sequence are often encountered. They can be purified from natural sources, appear as an unexpected fragment of a well characterized protein or as a contaminant. Regardless of the source of the problem, the unknown protein always requires tedious characterization. Here we present an automated pipeline for the identification of protein sequences from cryo-EM reconstructions and crystallographic data. We present the method’s application to characterize the crystal structure of an unknown protein purified from a snake venom. We also show that the approach can be successfully applied to the identification of protein sequences and validation of sequence assignments in cryo-EM protein structures..
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Preprint| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
bioRxiv.org - (2022) vom: 24. Dez. Zur Gesamtaufnahme - year:2022 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Chojnowski, Grzegorz [VerfasserIn] |
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| doi: |
10.1101/2021.04.18.440303 |
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| PPN (Katalog-ID): |
XBI020376243 |
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| 100 | 1 | |a Chojnowski, Grzegorz |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Identification of unknown proteins in X-ray crystallography and cryo-EM |
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| 520 | |a Abstract Although experimental protein structure determination usually targets known proteins, chains of unknown sequence are often encountered. They can be purified from natural sources, appear as an unexpected fragment of a well characterized protein or as a contaminant. Regardless of the source of the problem, the unknown protein always requires tedious characterization. Here we present an automated pipeline for the identification of protein sequences from cryo-EM reconstructions and crystallographic data. We present the method’s application to characterize the crystal structure of an unknown protein purified from a snake venom. We also show that the approach can be successfully applied to the identification of protein sequences and validation of sequence assignments in cryo-EM protein structures. | ||
| 650 | 4 | |a Biology |7 (dpeaa)DE-84 | |
| 650 | 4 | |a 570 |7 (dpeaa)DE-84 | |
| 700 | 1 | |a Simpkin, Adam J. |e verfasserin |4 aut | |
| 700 | 1 | |a Leonardo, Diego A. |e verfasserin |4 aut | |
| 700 | 1 | |a Seifert-Davila, Wolfram |e verfasserin |4 aut | |
| 700 | 1 | |a Vivas-Ruiz, Dan E. |e verfasserin |4 aut | |
| 700 | 1 | |a Keegan, Ronan M. |e verfasserin |4 aut | |
| 700 | 1 | |a Rigden, Daniel J. |e verfasserin |4 aut | |
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| 856 | 4 | 0 | |u http://dx.doi.org/10.1101/2021.04.18.440303 |z kostenfrei |3 Volltext |
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