Emergence of multiple SARS-CoV-2 mutations in an immunocompromised host
Abstract Prolonged shedding of infectious SARS-CoV-2 has recently been reported in a number of immunosuppressed individuals with COVID-19. Here, we describe the detection of high levels of replication-competent SARS-CoV-2 in specimens taken from the respiratory tract of a B-cell depleted patient up to 154 days after initial COVID-19 diagnosis concomitant with the development of high mutation rate. In this patient, a total of 11 nonsynonymous mutations were detected in addition to the Y144 deletion in the spike protein of SARS-CoV-2.Virus evolution studies revealed a dramatic diversification in viral population coinciding with treatment with convalescent plasma and clinical respiratory deterioration. Our findings highlight the urgent need for continuous real-time surveillance of genetic changes of SARS-CoV-2 adaptation alongside immunological investigations in patients with severely compromised humoral responses who may shed infectious virus over prolonged periods of time..
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Preprint| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
bioRxiv.org - (2021) vom: 17. Jan. Zur Gesamtaufnahme - year:2021 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Khatamzas, Elham [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.1101/2021.01.10.20248871 |
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| PPN (Katalog-ID): |
XBI019744927 |
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| 520 | |a Abstract Prolonged shedding of infectious SARS-CoV-2 has recently been reported in a number of immunosuppressed individuals with COVID-19. Here, we describe the detection of high levels of replication-competent SARS-CoV-2 in specimens taken from the respiratory tract of a B-cell depleted patient up to 154 days after initial COVID-19 diagnosis concomitant with the development of high mutation rate. In this patient, a total of 11 nonsynonymous mutations were detected in addition to the Y144 deletion in the spike protein of SARS-CoV-2.Virus evolution studies revealed a dramatic diversification in viral population coinciding with treatment with convalescent plasma and clinical respiratory deterioration. Our findings highlight the urgent need for continuous real-time surveillance of genetic changes of SARS-CoV-2 adaptation alongside immunological investigations in patients with severely compromised humoral responses who may shed infectious virus over prolonged periods of time. | ||
| 700 | 1 | |a Rehn, Alexandra |e verfasserin |4 aut | |
| 700 | 1 | |a Muenchhoff, Maximilian |e verfasserin |4 aut | |
| 700 | 1 | |a Hellmuth, Johannes |e verfasserin |4 aut | |
| 700 | 1 | |a Gaitzsch, Erik |e verfasserin |4 aut | |
| 700 | 1 | |a Weiglein, Tobias |e verfasserin |4 aut | |
| 700 | 1 | |a Georgi, Enrico |e verfasserin |4 aut | |
| 700 | 1 | |a Scherer, Clemens |e verfasserin |4 aut | |
| 700 | 1 | |a Stecher, Stephanie |e verfasserin |4 aut | |
| 700 | 1 | |a Weigert, Oliver |e verfasserin |4 aut | |
| 700 | 1 | |a Girl, Philipp |e verfasserin |4 aut | |
| 700 | 1 | |a Zange, Sabine |e verfasserin |4 aut | |
| 700 | 1 | |a Keppler, Oliver T. |e verfasserin |4 aut | |
| 700 | 1 | |a Stemmler, Joachim |e verfasserin |4 aut | |
| 700 | 1 | |a von Bergwelt-Baildon, Michael |e verfasserin |4 aut | |
| 700 | 1 | |a Wölfel, Roman |e verfasserin |4 aut | |
| 700 | 1 | |a Antwerpen, Markus |e verfasserin |4 aut | |
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