Muscle-specific Cavin4 interacts with Bin1 to promote T-tubule formation and stability in developing skeletal muscle
Summary The cavin proteins are essential for caveola biogenesis and function. Here, we identify a role for the muscle-specific component, Cavin4, in skeletal muscle T-tubule development by analyzing two vertebrate systems: mouse and zebrafish. In both models Cavin4 localized to T-tubules and loss of Cavin4 resulted in aberrant T-tubule maturation. In zebrafish, which possess duplicatedcavin4paralogs, Cavin4b was shown to directly interact with the T-tubule-associated BAR domain protein, Bin1. Loss of both Cavin4a and Cavin4b caused aberrant accumulation of interconnected caveolae within the T-tubules, a fragmented T-tubule network enriched in Caveolin-3, and an impaired Ca2+response upon mechanical stimulation. We propose a role for Cavin4 in remodeling the T-tubule membrane early in development by recycling caveolar components from the T-tubule to the sarcolemma. This generates a stable T-tubule domain lacking caveolae that is essential for T-tubule function..
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Preprint| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
bioRxiv.org - (2024) vom: 23. Apr. Zur Gesamtaufnahme - year:2024 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Lo, Harriet P. [VerfasserIn] |
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| doi: |
10.1101/2021.01.13.426456 |
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| PPN (Katalog-ID): |
XBI019744188 |
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| 100 | 1 | |a Lo, Harriet P. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Muscle-specific Cavin4 interacts with Bin1 to promote T-tubule formation and stability in developing skeletal muscle |
| 264 | 1 | |c 2024 | |
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| 520 | |a Summary The cavin proteins are essential for caveola biogenesis and function. Here, we identify a role for the muscle-specific component, Cavin4, in skeletal muscle T-tubule development by analyzing two vertebrate systems: mouse and zebrafish. In both models Cavin4 localized to T-tubules and loss of Cavin4 resulted in aberrant T-tubule maturation. In zebrafish, which possess duplicatedcavin4paralogs, Cavin4b was shown to directly interact with the T-tubule-associated BAR domain protein, Bin1. Loss of both Cavin4a and Cavin4b caused aberrant accumulation of interconnected caveolae within the T-tubules, a fragmented T-tubule network enriched in Caveolin-3, and an impaired Ca2+response upon mechanical stimulation. We propose a role for Cavin4 in remodeling the T-tubule membrane early in development by recycling caveolar components from the T-tubule to the sarcolemma. This generates a stable T-tubule domain lacking caveolae that is essential for T-tubule function. | ||
| 650 | 4 | |a Biology |7 (dpeaa)DE-84 | |
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| 700 | 1 | |a Lim, Ye-Wheen |e verfasserin |4 aut | |
| 700 | 1 | |a Xiong, Zherui |e verfasserin |4 aut | |
| 700 | 1 | |a Martel, Nick |e verfasserin |4 aut | |
| 700 | 1 | |a Ferguson, Charles |e verfasserin |4 aut | |
| 700 | 1 | |a Ariotti, Nicholas R. |e verfasserin |4 aut | |
| 700 | 1 | |a Giacomotto, Jean |e verfasserin |4 aut | |
| 700 | 1 | |a Rae, James A. |e verfasserin |4 aut | |
| 700 | 1 | |a Floetenmeyer, Matthias |e verfasserin |4 aut | |
| 700 | 1 | |a Moradi, Shayli Varasteh |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, Ya |e verfasserin |4 aut | |
| 700 | 1 | |a Tillu, Vikas A. |e verfasserin |4 aut | |
| 700 | 1 | |a Xia, Di |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Huang |e verfasserin |4 aut | |
| 700 | 1 | |a Rahnama, Samira |e verfasserin |4 aut | |
| 700 | 1 | |a Nixon, Susan J. |e verfasserin |4 aut | |
| 700 | 1 | |a Bastiani, Michele |e verfasserin |4 aut | |
| 700 | 1 | |a Day, Ryan D. |e verfasserin |4 aut | |
| 700 | 1 | |a Smith, Kelly A. |e verfasserin |4 aut | |
| 700 | 1 | |a Palpant, Nathan J. |e verfasserin |4 aut | |
| 700 | 1 | |a Johnston, Wayne A. |e verfasserin |4 aut | |
| 700 | 1 | |a Alexandrov, Kirill |e verfasserin |4 aut | |
| 700 | 1 | |a Collins, Brett M. |e verfasserin |4 aut | |
| 700 | 1 | |a Hall, Thomas E. |e verfasserin |4 aut | |
| 700 | 1 | |a Parton, Robert G. |e verfasserin |4 aut | |
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