Genome-scale sequencing and analysis of human, wolf and bison DNA from 25,000 year-old sediment
Summary Archaeological sediments have been shown to preserve ancient DNA, but so far have not yielded genome-scale information of the magnitude of skeletal remains. We retrieved and analysed human and mammalian low-coverage nuclear and high-coverage mitochondrial genomes from Upper Palaeolithic sediments from Satsurblia cave, western Georgia, dated to 25,000 years ago. First, a human female genome with substantial basal Eurasian ancestry, which was an ancestry component of the majority of post-Ice Age people in the Near East, North Africa, and parts of Europe. Second, a wolf genome that is basal to extant Eurasian wolves and dogs and represents a previously unknown, likely extinct, Caucasian lineage that diverged from the ancestors of modern wolves and dogs before these diversified. Third, a bison genome that is basal to present-day populations, suggesting that population structure has been substantially reshaped since the Last Glacial Maximum. Our results provide new insights into the late Pleistocene genetic histories of these three species, and demonstrate that sediment DNA can be used not only for species identification, but also be a source of genome-wide ancestry information and genetic history.Highlights <jats:list list-type="bullet">We demonstrate for the first time that genome sequencing from sediments is comparable to that of skeletal remainsA single Pleistocene sediment sample from the Caucasus yielded three low-coverage mammalian ancient genomesWe show that sediment ancient DNA can reveal important aspects of the human and faunal pastEvidence of an uncharacterized human lineage from the Caucasus before the Last Glacial Maximum∼0.01-fold coverage wolf and bison genomes are both basal to present-day diversity, suggesting reshaping of population structure in both species.
| Medienart: |
|
|---|
Preprint| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
bioRxiv.org - (2021) vom: 15. Dez. Zur Gesamtaufnahme - year:2021 |
|---|
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Gelabert, Pere [VerfasserIn] |
|---|
| Links: |
|---|
| doi: |
10.1101/2021.01.08.425895 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
XBI019700504 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | XBI019700504 | ||
| 003 | DE-627 | ||
| 005 | 20230429094708.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 210111s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1101/2021.01.08.425895 |2 doi | |
| 035 | |a (DE-627)XBI019700504 | ||
| 035 | |a (biorXiv)10.1101/2021.01.08.425895 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | |a 570 |q DE-84 | |
| 100 | 1 | |a Gelabert, Pere |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Genome-scale sequencing and analysis of human, wolf and bison DNA from 25,000 year-old sediment |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a Summary Archaeological sediments have been shown to preserve ancient DNA, but so far have not yielded genome-scale information of the magnitude of skeletal remains. We retrieved and analysed human and mammalian low-coverage nuclear and high-coverage mitochondrial genomes from Upper Palaeolithic sediments from Satsurblia cave, western Georgia, dated to 25,000 years ago. First, a human female genome with substantial basal Eurasian ancestry, which was an ancestry component of the majority of post-Ice Age people in the Near East, North Africa, and parts of Europe. Second, a wolf genome that is basal to extant Eurasian wolves and dogs and represents a previously unknown, likely extinct, Caucasian lineage that diverged from the ancestors of modern wolves and dogs before these diversified. Third, a bison genome that is basal to present-day populations, suggesting that population structure has been substantially reshaped since the Last Glacial Maximum. Our results provide new insights into the late Pleistocene genetic histories of these three species, and demonstrate that sediment DNA can be used not only for species identification, but also be a source of genome-wide ancestry information and genetic history.Highlights <jats:list list-type="bullet">We demonstrate for the first time that genome sequencing from sediments is comparable to that of skeletal remainsA single Pleistocene sediment sample from the Caucasus yielded three low-coverage mammalian ancient genomesWe show that sediment ancient DNA can reveal important aspects of the human and faunal pastEvidence of an uncharacterized human lineage from the Caucasus before the Last Glacial Maximum∼0.01-fold coverage wolf and bison genomes are both basal to present-day diversity, suggesting reshaping of population structure in both species | ||
| 700 | 1 | |a Sawyer, Susanna |e verfasserin |4 aut | |
| 700 | 1 | |a Bergström, Anders |e verfasserin |4 aut | |
| 700 | 1 | |a Collin, Thomas C. |e verfasserin |4 aut | |
| 700 | 1 | |a Meshveliani, Tengiz |e verfasserin |4 aut | |
| 700 | 1 | |a Belfer-Cohen, Anna |e verfasserin |4 aut | |
| 700 | 1 | |a Lordkipanidze, David |e verfasserin |4 aut | |
| 700 | 1 | |a Jakeli, Nino |e verfasserin |4 aut | |
| 700 | 1 | |a Matskevich, Zinovi |e verfasserin |4 aut | |
| 700 | 1 | |a Bar-Oz, Guy |e verfasserin |4 aut | |
| 700 | 1 | |a Fernandes, Daniel M. |e verfasserin |4 aut | |
| 700 | 1 | |a Cheronet, Olivia |e verfasserin |4 aut | |
| 700 | 1 | |a Özdoğan, Kadir T. |e verfasserin |4 aut | |
| 700 | 1 | |a Oberreiter, Victoria |e verfasserin |4 aut | |
| 700 | 1 | |a Feeney, Robin N. M. |e verfasserin |4 aut | |
| 700 | 1 | |a Stahlschmidt, Mareike C. |e verfasserin |4 aut | |
| 700 | 1 | |a Skoglund, Pontus |e verfasserin |4 aut | |
| 700 | 1 | |a Pinhasi, Ron |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t bioRxiv.org |g (2021) vom: 15. Dez. |
| 773 | 1 | 8 | |g year:2021 |g day:15 |g month:12 |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.cub.2021.06.023 |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1101/2021.01.08.425895 |z kostenfrei |3 Volltext |
| 912 | |a GBV_XBI | ||
| 912 | |a SSG-OLC-PHA | ||
| 951 | |a AR | ||
| 952 | |j 2021 |b 15 |c 12 | ||
| 953 | |2 045F |a 570 | ||