Extracellular spreading of Wingless is required for<i>Drosophila</i>oogenesis
Abstract Recent studies have investigated whether the Wnt family of extracellular ligands can signal at long range, spreading from their source and acting as morphogens, or whether they signal only in a juxtacrine manner to neighboring cells. The original evidence for long-range Wnt signaling arose from studies of Wg, aDrosophilaWnt protein, which patterns the wing disc over several cell diameters from a central source of Wg ligand. However, the requirement of long-range Wg for patterning was called into question when it was reported that replacing the secreted protein Wg with a membrane-tethered version, NRT-Wg, results in flies with normally patterned wings. We and others previously reported that Wg spreads in the ovary about 50 μm or 5 cell diameters, from the cap cells to the follicle stem cells (FSCs) and that Wg stimulates FSC proliferation. We used theNRT-wgflies to analyze the consequence of tethering Wg to the cap cells.NRT-wghomozygous flies are sickly, but we found that hemizygousNRT-wg/nullflies, carrying only one copy of tethered Wingless, were significantly healthier. Despite their overall improved health, these hemizygous flies displayed dramatic reductions in fertility and in FSC proliferation. Further, FSC proliferation was nearly undetectable when thewglocus was converted toNRT-wgonly in adults, and the resulting germarium phenotype was consistent with a previously reportedwgloss-of-function phenotype. We conclude that Wg protein spreads from its source cells in the germarium to promote FSC proliferation..
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Preprint| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
bioRxiv.org - (2024) vom: 23. Apr. Zur Gesamtaufnahme - year:2024 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wang, Xiaoxi [VerfasserIn] |
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| doi: |
10.1101/2020.12.08.416594 |
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| PPN (Katalog-ID): |
XBI019523203 |
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| 520 | |a Abstract Recent studies have investigated whether the Wnt family of extracellular ligands can signal at long range, spreading from their source and acting as morphogens, or whether they signal only in a juxtacrine manner to neighboring cells. The original evidence for long-range Wnt signaling arose from studies of Wg, aDrosophilaWnt protein, which patterns the wing disc over several cell diameters from a central source of Wg ligand. However, the requirement of long-range Wg for patterning was called into question when it was reported that replacing the secreted protein Wg with a membrane-tethered version, NRT-Wg, results in flies with normally patterned wings. We and others previously reported that Wg spreads in the ovary about 50 μm or 5 cell diameters, from the cap cells to the follicle stem cells (FSCs) and that Wg stimulates FSC proliferation. We used theNRT-wgflies to analyze the consequence of tethering Wg to the cap cells.NRT-wghomozygous flies are sickly, but we found that hemizygousNRT-wg/nullflies, carrying only one copy of tethered Wingless, were significantly healthier. Despite their overall improved health, these hemizygous flies displayed dramatic reductions in fertility and in FSC proliferation. Further, FSC proliferation was nearly undetectable when thewglocus was converted toNRT-wgonly in adults, and the resulting germarium phenotype was consistent with a previously reportedwgloss-of-function phenotype. We conclude that Wg protein spreads from its source cells in the germarium to promote FSC proliferation. | ||
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