ELOVL2 is required for robust visual function in zebrafish
ABSTRACT Omega-3 and omega-6 polyunsaturated fatty acids (PUFAs) play critical roles in membrane stability and cell signaling within the retina.Elovl2, an elongase involved in synthesis of long chain polyunsaturated fatty acids (LC-PUFAs), has recently been implicated in regulating aging in the mammalian retina. In this work, we characterize the expression and function ofelovl2in retina development in embryonic zebrafish. Whole mountin situhybridization showselovl2is expressed in the Müller glia in embryonic and adult zebrafish. Lipidomics analysis ofelovl2crispants whole embryos at day 2 and eyes at day 7 demonstrated significant changes in lipids composition, especially on the level of lipids containing docosahexaenoic acid (DHA). Histological analysis of zebrafish lackingelovl2revealed increased retinal thickness compared to controls at day 7 without gross disruptions of retinal architecture. Finally,elovl2crispants showed differences in the visual motor reflex light off (VMR-OFF) at day 7 compared to controls. In sum, inactivation ofelovl2in zebrafish embryos caused changes in lipid composition and in visual behavior further confirming the important role of LC-PUFAs in healthy vision..
| Medienart: |
|
|---|
Preprint| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
bioRxiv.org - (2023) vom: 09. Okt. Zur Gesamtaufnahme - year:2023 |
|---|
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Dasyani, Manish [VerfasserIn] |
|---|
| Links: |
Volltext [kostenfrei] |
|---|
| Themen: |
|---|
| doi: |
10.1101/2020.10.13.336974 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
XBI019130821 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | XBI019130821 | ||
| 003 | DE-627 | ||
| 005 | 20231205150439.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 201016s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1101/2020.10.13.336974 |2 doi | |
| 035 | |a (DE-627)XBI019130821 | ||
| 035 | |a (biorXiv)10.1101/2020.10.13.336974 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Dasyani, Manish |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a ELOVL2 is required for robust visual function in zebrafish |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a ABSTRACT Omega-3 and omega-6 polyunsaturated fatty acids (PUFAs) play critical roles in membrane stability and cell signaling within the retina.Elovl2, an elongase involved in synthesis of long chain polyunsaturated fatty acids (LC-PUFAs), has recently been implicated in regulating aging in the mammalian retina. In this work, we characterize the expression and function ofelovl2in retina development in embryonic zebrafish. Whole mountin situhybridization showselovl2is expressed in the Müller glia in embryonic and adult zebrafish. Lipidomics analysis ofelovl2crispants whole embryos at day 2 and eyes at day 7 demonstrated significant changes in lipids composition, especially on the level of lipids containing docosahexaenoic acid (DHA). Histological analysis of zebrafish lackingelovl2revealed increased retinal thickness compared to controls at day 7 without gross disruptions of retinal architecture. Finally,elovl2crispants showed differences in the visual motor reflex light off (VMR-OFF) at day 7 compared to controls. In sum, inactivation ofelovl2in zebrafish embryos caused changes in lipid composition and in visual behavior further confirming the important role of LC-PUFAs in healthy vision. | ||
| 650 | 4 | |a Biology |7 (dpeaa)DE-84 | |
| 650 | 4 | |a 570 |7 (dpeaa)DE-84 | |
| 700 | 1 | |a Gao, Fangyuan |4 aut | |
| 700 | 1 | |a Xu, Qianlan |4 aut | |
| 700 | 1 | |a Fossan, Donald Van |4 aut | |
| 700 | 1 | |a Zhang, Ellis |4 aut | |
| 700 | 1 | |a Pinto, Antonio F. M. |4 aut | |
| 700 | 1 | |a Saghatelian, Alan |4 aut | |
| 700 | 1 | |a Skowronska-Krawczyk, Dorota |0 (orcid)0000-0002-5758-4225 |4 aut | |
| 700 | 1 | |a Chao, Daniel L. |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t bioRxiv.org |g (2023) vom: 09. Okt. |
| 773 | 1 | 8 | |g year:2023 |g day:09 |g month:10 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1101/2020.10.13.336974 |z kostenfrei |3 Volltext |
| 912 | |a GBV_XBI | ||
| 951 | |a AR | ||
| 952 | |j 2023 |b 09 |c 10 | ||