Rapid “mix and read” assay for scalable detection of SARS-CoV-2 antibodies in patient plasma
Abstract The human beta coronavirus SARS-CoV-2, causative virus of COVID-19, has infected more than 15 million people globally and continues to spread. Widespread, population level testing to detect active and past infections is critical to curb the COVID-19 pandemic. Antibody (serological) testing is the only option for detecting past infections outside the narrow window accessible to nucleic acid-based tests. However, currently available serological assays commonly lack scalability. Here, we describe the development of a rapid homogenous serological assay for the detection of antibodies to SARS-CoV-2 in patient plasma. We show that the fluorescence-based assay accurately detects seroconversion in COVID-19 patients from less than 1μL of plasma. Using a cohort of samples from COVID-19 infected or healthy individuals, we demonstrate detection with 100% sensitivity and specificity. This assay addresses an important need for a robust, low barrier to implementation, and scalable serological assay with complementary strengths to currently available serological platforms..
| Medienart: |
|
|---|
Preprint| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
bioRxiv.org - (2024) vom: 23. Apr. Zur Gesamtaufnahme - year:2024 |
|---|
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Yue, Hong [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
|---|
| doi: |
10.1101/2020.09.01.20184101 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
XBI018674836 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | XBI018674836 | ||
| 003 | DE-627 | ||
| 005 | 20240425104657.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200904s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1101/2020.09.01.20184101 |2 doi | |
| 035 | |a (DE-627)XBI018674836 | ||
| 035 | |a (biorXiv)10.1101/2020.09.01.20184101 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Yue, Hong |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Rapid “mix and read” assay for scalable detection of SARS-CoV-2 antibodies in patient plasma |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a Abstract The human beta coronavirus SARS-CoV-2, causative virus of COVID-19, has infected more than 15 million people globally and continues to spread. Widespread, population level testing to detect active and past infections is critical to curb the COVID-19 pandemic. Antibody (serological) testing is the only option for detecting past infections outside the narrow window accessible to nucleic acid-based tests. However, currently available serological assays commonly lack scalability. Here, we describe the development of a rapid homogenous serological assay for the detection of antibodies to SARS-CoV-2 in patient plasma. We show that the fluorescence-based assay accurately detects seroconversion in COVID-19 patients from less than 1μL of plasma. Using a cohort of samples from COVID-19 infected or healthy individuals, we demonstrate detection with 100% sensitivity and specificity. This assay addresses an important need for a robust, low barrier to implementation, and scalable serological assay with complementary strengths to currently available serological platforms. | ||
| 650 | 4 | |a Biology |7 (dpeaa)DE-84 | |
| 650 | 4 | |a 570 |7 (dpeaa)DE-84 | |
| 700 | 1 | |a Nowak, Radosław P. |e verfasserin |0 (orcid)0000-0002-0605-0071 |4 aut | |
| 700 | 1 | |a Overwijn, Daan |e verfasserin |4 aut | |
| 700 | 1 | |a Payne, N. Connor |e verfasserin |4 aut | |
| 700 | 1 | |a Fischinger, Stephanie |e verfasserin |0 (orcid)0000-0003-2307-3379 |4 aut | |
| 700 | 1 | |a Atyeo, Caroline |e verfasserin |4 aut | |
| 700 | 1 | |a Baden, Lindsey R. |e verfasserin |4 aut | |
| 700 | 1 | |a Nilles, Eric J. |e verfasserin |0 (orcid)0000-0001-7044-5257 |4 aut | |
| 700 | 1 | |a Karlson, Elizabeth W. |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Xu G. |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Jonathan Z. |e verfasserin |4 aut | |
| 700 | 1 | |a Alter, Galit |e verfasserin |4 aut | |
| 700 | 1 | |a Mazitschek, Ralph |e verfasserin |4 aut | |
| 700 | 1 | |a Fischer, Eric S. |e verfasserin |0 (orcid)0000-0001-7337-6306 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t bioRxiv.org |g (2024) vom: 23. Apr. |
| 773 | 1 | 8 | |g year:2024 |g day:23 |g month:04 |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.crmeth.2023.100421 |x 0 |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1101/2020.09.01.20184101 |x 0 |z kostenfrei |3 Volltext |
| 912 | |a GBV_XBI | ||
| 951 | |a AR | ||
| 952 | |j 2024 |b 23 |c 04 | ||