G protein subunit gamma 4 expression has potential of detection, prediction, and therapeutic target for liver metastasis of gastric cancer
ABSTRACT Liver metastasis of gastric cancer is the most common for hematogenous metastases and so fatal, that the identification of novel markers and targets for therapy are crucial. We conducted transcriptome analyses between synchronous liver metastasis, primary tumor, and adjacent tissues from four patients with metastasis confined to the liver to discover thatGNG4upregulated substantially in primary gastric cancer tissues. Quantitative RT-qPCR assay for 300 gastric cancer patients revealed that higher levels ofGNG4in primary cancer were associated with shorter overall survival and a higher risk of liver recurrence. The oncogenic phenotypes ofGNG4were determined by knockout and forced expression ofGNG4. Tumor formation byGNG4knockout cells was more strikingly attenuated in a liver metastasis mouse model compared with a subcutaneous model.GNG4is a candidate for a therapeutic target for liver metastasis, and its expression may enable us to provide better disease monitoring for liver metastasis..
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Preprint| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
bioRxiv.org - (2022) vom: 07. Nov. Zur Gesamtaufnahme - year:2022 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Tanaka, Haruyoshi [VerfasserIn] |
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| doi: |
10.1101/2020.08.14.20175034 |
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| PPN (Katalog-ID): |
XBI018618685 |
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| 100 | 1 | |a Tanaka, Haruyoshi |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a G protein subunit gamma 4 expression has potential of detection, prediction, and therapeutic target for liver metastasis of gastric cancer |
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| 520 | |a ABSTRACT Liver metastasis of gastric cancer is the most common for hematogenous metastases and so fatal, that the identification of novel markers and targets for therapy are crucial. We conducted transcriptome analyses between synchronous liver metastasis, primary tumor, and adjacent tissues from four patients with metastasis confined to the liver to discover thatGNG4upregulated substantially in primary gastric cancer tissues. Quantitative RT-qPCR assay for 300 gastric cancer patients revealed that higher levels ofGNG4in primary cancer were associated with shorter overall survival and a higher risk of liver recurrence. The oncogenic phenotypes ofGNG4were determined by knockout and forced expression ofGNG4. Tumor formation byGNG4knockout cells was more strikingly attenuated in a liver metastasis mouse model compared with a subcutaneous model.GNG4is a candidate for a therapeutic target for liver metastasis, and its expression may enable us to provide better disease monitoring for liver metastasis. | ||
| 650 | 4 | |a Biology |7 (dpeaa)DE-84 | |
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| 700 | 1 | |a Kanda, Mitsuro |e verfasserin |4 aut | |
| 700 | 1 | |a Miwa, Takashi |e verfasserin |4 aut | |
| 700 | 1 | |a Umeda, Shinichi |e verfasserin |4 aut | |
| 700 | 1 | |a Sawaki, Koichi |e verfasserin |4 aut | |
| 700 | 1 | |a Tanaka, Chie |e verfasserin |4 aut | |
| 700 | 1 | |a Kobayashi, Daisuke |e verfasserin |4 aut | |
| 700 | 1 | |a Hayashi, Masamichi |e verfasserin |4 aut | |
| 700 | 1 | |a Yamada, Suguru |e verfasserin |4 aut | |
| 700 | 1 | |a Nakayama, Goro |e verfasserin |4 aut | |
| 700 | 1 | |a Koike, Masahiko |e verfasserin |4 aut | |
| 700 | 1 | |a Kodera, Yasuhiro |e verfasserin |4 aut | |
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