cDC1 Coordinate Innate and Adaptive Responses in the Omentum required for T cell Priming and Memory
ABSTRACT The omentum in the peritoneal cavity contains fat associated lymphoid clusters (FALCs) whose role in the response to microbial challenge are poorly understood. After intraperitoneal immunization withToxoplasma gondii, type I dendritic cells (cDC1) were critical to induce innate sources of IFN-γ required to recruit monocytes to the FALCs. The migration of infected peritoneal macrophages into T and B cell rich areas of the FALCs allowed the TCR-induced activation of parasite-specific T cells. Unexpectedly, cDC1 were not required for T cell priming but rather supported the expansion of parasite-specific CD8+T cells. An agent-based mathematical model predicted that the lack of cDC1 would impact the early proliferative burst, and we confirmed that cDC1 were required for optimal T cell expression of nutrient uptake receptors and cell survival. These studies highlight that cDC1 in the FALCs have distinct roles in the co-ordination of the innate and adaptive responses to microbial challenge..
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Preprint| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
bioRxiv.org - (2023) vom: 04. Okt. Zur Gesamtaufnahme - year:2023 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Christian, David A. [VerfasserIn] |
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| doi: |
10.1101/2020.07.21.214809 |
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| PPN (Katalog-ID): |
XBI018394620 |
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| 245 | 1 | 0 | |a cDC1 Coordinate Innate and Adaptive Responses in the Omentum required for T cell Priming and Memory |
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| 520 | |a ABSTRACT The omentum in the peritoneal cavity contains fat associated lymphoid clusters (FALCs) whose role in the response to microbial challenge are poorly understood. After intraperitoneal immunization withToxoplasma gondii, type I dendritic cells (cDC1) were critical to induce innate sources of IFN-γ required to recruit monocytes to the FALCs. The migration of infected peritoneal macrophages into T and B cell rich areas of the FALCs allowed the TCR-induced activation of parasite-specific T cells. Unexpectedly, cDC1 were not required for T cell priming but rather supported the expansion of parasite-specific CD8+T cells. An agent-based mathematical model predicted that the lack of cDC1 would impact the early proliferative burst, and we confirmed that cDC1 were required for optimal T cell expression of nutrient uptake receptors and cell survival. These studies highlight that cDC1 in the FALCs have distinct roles in the co-ordination of the innate and adaptive responses to microbial challenge. | ||
| 650 | 4 | |a Biology |7 (dpeaa)DE-84 | |
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| 700 | 1 | |a Adams, Thomas A. |4 aut | |
| 700 | 1 | |a Smith, Tony E. |4 aut | |
| 700 | 1 | |a Shallberg, Lindsey A. |0 (orcid)0000-0001-6573-7327 |4 aut | |
| 700 | 1 | |a Theisen, Derek J. |0 (orcid)0000-0002-2204-8676 |4 aut | |
| 700 | 1 | |a Phan, Anthony T. |0 (orcid)0000-0003-2935-6070 |4 aut | |
| 700 | 1 | |a Abraha, Mosana |4 aut | |
| 700 | 1 | |a Perry, Joseph |4 aut | |
| 700 | 1 | |a Ruthel, Gordon |4 aut | |
| 700 | 1 | |a Clark, Joseph T. |0 (orcid)0000-0001-7764-6000 |4 aut | |
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| 700 | 1 | |a Kedl, Ross M. |0 (orcid)0000-0002-9065-7895 |4 aut | |
| 700 | 1 | |a Hunter, Christopher A. |0 (orcid)0000-0003-3092-1428 |4 aut | |
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