Single-cell transcriptomic analysis of SARS-CoV-2 reactive CD4<sup>+</sup>T cells
ABSTRACT The contribution of CD4+T cells to protective or pathogenic immune responses to SARS-CoV-2 infection remains unknown. Here, we present large-scale single-cell transcriptomic analysis of viral antigen-reactive CD4+T cells from 32 COVID-19 patients. In patients with severe disease compared to mild disease, we found increased proportions of cytotoxic follicular helper (TFH) cells and cytotoxic T helper cells (CD4-CTLs) responding to SARS-CoV-2, and reduced proportion of SARS-CoV-2 reactive regulatory T cells. Importantly, the CD4-CTLs were highly enriched for the expression of transcripts encoding chemokines that are involved in the recruitment of myeloid cells and dendritic cells to the sites of viral infection. Polyfunctional T helper (TH)1 cells and TH17 cell subsets were underrepresented in the repertoire of SARS-CoV-2-reactive CD4+T cells compared to influenza-reactive CD4+T cells. Together, our analyses provide so far unprecedented insights into the gene expression patterns of SARS-CoV-2 reactive CD4+T cells in distinct disease severities..
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Preprint| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
bioRxiv.org - (2022) vom: 28. Okt. Zur Gesamtaufnahme - year:2022 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Meckiff, Benjamin J. [VerfasserIn] |
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| doi: |
10.1101/2020.06.12.148916 |
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| PPN (Katalog-ID): |
XBI018128297 |
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| 520 | |a ABSTRACT The contribution of CD4+T cells to protective or pathogenic immune responses to SARS-CoV-2 infection remains unknown. Here, we present large-scale single-cell transcriptomic analysis of viral antigen-reactive CD4+T cells from 32 COVID-19 patients. In patients with severe disease compared to mild disease, we found increased proportions of cytotoxic follicular helper (TFH) cells and cytotoxic T helper cells (CD4-CTLs) responding to SARS-CoV-2, and reduced proportion of SARS-CoV-2 reactive regulatory T cells. Importantly, the CD4-CTLs were highly enriched for the expression of transcripts encoding chemokines that are involved in the recruitment of myeloid cells and dendritic cells to the sites of viral infection. Polyfunctional T helper (TH)1 cells and TH17 cell subsets were underrepresented in the repertoire of SARS-CoV-2-reactive CD4+T cells compared to influenza-reactive CD4+T cells. Together, our analyses provide so far unprecedented insights into the gene expression patterns of SARS-CoV-2 reactive CD4+T cells in distinct disease severities. | ||
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| 700 | 1 | |a Chee, Serena J |e verfasserin |4 aut | |
| 700 | 1 | |a Kusnadi, Anthony |e verfasserin |4 aut | |
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| 700 | 1 | |a Grifoni, Alba |e verfasserin |4 aut | |
| 700 | 1 | |a Pelosi, Emanuela |e verfasserin |4 aut | |
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| 700 | 1 | |a Ottensmeier, Christian H |e verfasserin |4 aut | |
| 700 | 1 | |a Vijayanand, Pandurangan |e verfasserin |4 aut | |
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