Novel R-type Lectin Domain-Containing Cytotoxins Comprise a Family of Virulence-Modifying Proteins in PathogenicLeptospira

Abstract Leptospirosis is a globally important neglected zoonotic disease subject to both small scale outbreaks and weather-driven, large-scale epidemics. Due to gaps in our understanding ofLeptospirabiology, pathogenetic mechanisms of leptospirosis remain largely unknown. Previous data suggest that a gene family, PF07598, unique amongst most known bacterial pathogens and encoding so-called “Virulence-Modifying (VM)” proteins, are important virulence determinants. Here, we show that VM proteins are potent cytotoxins, sharing a distinct domain organization while exhibiting varied mechanisms of cellular toxicity. Structural homology searches using Phyre2 suggest that VM proteins are novel R-type lectins containing an N-terminal ricin B chain-like domain. As is known for native ricin B-chain, recombinant full-lengthrLA3490(most highly up-regulatedin vivo) and an N-terminal fragment,t3490, containing a partial ricin B-domain, bound to asialofetuin and directly competed for asialofetuin binding with recombinant ricin B chain. Whilet3490bound to the HeLa cell surface but was neither internalized nor cytotoxic,rLA3490bound to the HeLa cell surface, was rapidly internalized, translocated to the nucleus inducing chromosomal fragmentation, and was rapidly cytolethal, providing strong evidence thatLeptospiraVM proteins arebona fidecytotoxins. Because monoclonal antibodies impeding cell entry or intracellular trafficking of ricin holotoxin clearly mitigate its toxicity, that VM proteins share binding and intracellular trafficking mechanisms suggests that anti-VM-protein antibody-based (anti-toxin) therapeutics could ameliorate severe complications of leptospirosis thereby improving prognosis. As most VM proteins are restricted to high-virulenceLeptospiraspecies with some, e.g., LA3490, being exceptionally potent, their level in serum might be a potentially useful indicator of a poor prognosis, thus identifying high risk patients.Author Summary The PF07598 gene family encoding Virulence-Modifying (VM) proteins in pathogenicLeptospiraspecies is associated with severe manifestations of leptospirosis. Structural homology searches indicate that VM proteins contain an N-terminal ricin B chain-like domain, biochemically confirmed in asialofetuin binding and competitive-binding assays suggesting that VM proteins bind to terminal galactosyl residues of this model ricin B domain binding protein. The leptospiral N-terminal ricin B chain-like domain mediated VM protein binding to HeLa cells. Full-length recombinant protein rapidly led to cell death. Amino acid conservation among PF07598 family members at the N-terminal ricin B chain-like domain suggests that VM protein levels in serum might be a useful biomarker for quickly identifying at-risk patients, and that novel “anti-toxin”-based therapeutics could ameliorate severe complications of leptospirosis, both of which remain to be explored..

Medienart:	Preprint

Erscheinungsjahr:	2021
Erschienen:	2021

Enthalten in:	bioRxiv.org - (2021) vom: 14. März Zur Gesamtaufnahme - year:2021

Sprache:	Englisch

Beteiligte Personen:	Chaurasia, Reetika [VerfasserIn] Marroquin, Alan [VerfasserIn] Matthias, Michael A. [VerfasserIn] Vinetz, Joseph M. [VerfasserIn]

Links:	Volltext [kostenfrei]

doi:	10.1101/2020.05.13.094169

funding:
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PPN (Katalog-ID):	XBI017902142