Self-amplifying RNA SARS-CoV-2 lipid nanoparticle vaccine induces equivalent preclinical antibody titers and viral neutralization to recovered COVID-19 patients
ABSTRACT The spread of the SARS-CoV-2 into a global pandemic within a few months of onset motivates the development of a rapidly scalable vaccine. Here, we present a self-amplifying RNA encoding the SARS-CoV-2 spike protein encapsulated within a lipid nanoparticle as a vaccine and demonstrate induction of robust neutralization of a pseudo-virus, proportional to quantity of specific IgG and of higher quantities than recovered COVID-19 patients. These data provide insight into the vaccine design and evaluation of immunogenicity to enable rapid translation to the clinic..
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Preprint| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
bioRxiv.org - (2023) vom: 30. Sept. Zur Gesamtaufnahme - year:2023 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
McKay, Paul F. [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.1101/2020.04.22.055608 |
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| PPN (Katalog-ID): |
XBI017743982 |
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| 245 | 1 | 0 | |a Self-amplifying RNA SARS-CoV-2 lipid nanoparticle vaccine induces equivalent preclinical antibody titers and viral neutralization to recovered COVID-19 patients |
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| 520 | |a ABSTRACT The spread of the SARS-CoV-2 into a global pandemic within a few months of onset motivates the development of a rapidly scalable vaccine. Here, we present a self-amplifying RNA encoding the SARS-CoV-2 spike protein encapsulated within a lipid nanoparticle as a vaccine and demonstrate induction of robust neutralization of a pseudo-virus, proportional to quantity of specific IgG and of higher quantities than recovered COVID-19 patients. These data provide insight into the vaccine design and evaluation of immunogenicity to enable rapid translation to the clinic. | ||
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| 700 | 1 | |a Hu, Kai |4 aut | |
| 700 | 1 | |a Blakney, Anna K. |4 aut | |
| 700 | 1 | |a Samnuan, Karnyart |4 aut | |
| 700 | 1 | |a Bouton, Clément R. |4 aut | |
| 700 | 1 | |a Rogers, Paul |4 aut | |
| 700 | 1 | |a Polra, Krunal |4 aut | |
| 700 | 1 | |a Lin, Paulo J.C. |4 aut | |
| 700 | 1 | |a Barbosa, Christopher |4 aut | |
| 700 | 1 | |a Tam, Ying |4 aut | |
| 700 | 1 | |a Shattock, Robin J. |4 aut | |
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