Mechanism of action of HBV capsid assembly modulators predicted from binding to early assembly intermediates
ABSTRACT Interfering with the self-assembly of virus nucleocapsids is a promising approach for the development of novel antiviral agents. Applied to hepatitis B virus (HBV), this approach has led to several classes of capsid assembly modulators (CAMs) that target the virus by either accelerating nucleocapsid assembly or misdirecting it into non-capsid-like particles. Here, we have assessed the structures of early nucleocapsid assembly intermediates, with and without bound CAMs, using molecular dynamics simulations. We find that distinct conformations of the intermediates are induced depending on whether the bound CAM accelerates or misdirects assembly; these structures are predictive of the final assembly. We also selected non-capsid-like structures from our simulations for virtual screening, resulting in the discovery of several compounds with moderate anti-viral activity and low toxicity. Cryo-electron microscopy and capsid melting experiments suggest that our compounds possess a novel mechanism for assembly modulation, potentially opening new avenues for HBV inhibition..
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Preprint| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
bioRxiv.org - (2020) vom: 28. März Zur Gesamtaufnahme - year:2020 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Pavlova, Anna [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.1101/2020.03.23.002527 |
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| PPN (Katalog-ID): |
XBI000816124 |
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| 245 | 1 | 0 | |a Mechanism of action of HBV capsid assembly modulators predicted from binding to early assembly intermediates |
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| 520 | |a ABSTRACT Interfering with the self-assembly of virus nucleocapsids is a promising approach for the development of novel antiviral agents. Applied to hepatitis B virus (HBV), this approach has led to several classes of capsid assembly modulators (CAMs) that target the virus by either accelerating nucleocapsid assembly or misdirecting it into non-capsid-like particles. Here, we have assessed the structures of early nucleocapsid assembly intermediates, with and without bound CAMs, using molecular dynamics simulations. We find that distinct conformations of the intermediates are induced depending on whether the bound CAM accelerates or misdirects assembly; these structures are predictive of the final assembly. We also selected non-capsid-like structures from our simulations for virtual screening, resulting in the discovery of several compounds with moderate anti-viral activity and low toxicity. Cryo-electron microscopy and capsid melting experiments suggest that our compounds possess a novel mechanism for assembly modulation, potentially opening new avenues for HBV inhibition. | ||
| 700 | 1 | |a Bassit, Leda |e verfasserin |4 aut | |
| 700 | 1 | |a Cox, Bryan D. |e verfasserin |4 aut | |
| 700 | 1 | |a Korablyov, Maksym |e verfasserin |4 aut | |
| 700 | 1 | |a Chipot, Chris |e verfasserin |4 aut | |
| 700 | 1 | |a Verma, Kiran |e verfasserin |4 aut | |
| 700 | 1 | |a Russell, Olivia O. |e verfasserin |4 aut | |
| 700 | 1 | |a Schinazi, Raymond F. |e verfasserin |4 aut | |
| 700 | 1 | |a Gumbart, James C. |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t bioRxiv.org |g (2020) vom: 28. März |
| 773 | 1 | 8 | |g year:2020 |g day:28 |g month:03 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1101/2020.03.23.002527 |z kostenfrei |3 Volltext |
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