GREM1 is epigenetically reprogrammed in muscle cells after exercise training and controls myogenesis and metabolism
Abstract Exercise training improves skeletal muscle function, notably through tissue regeneration by muscle stem cells. Here, we hypothesized that exercise training reprograms the epigenome of muscle cell, which could account for better muscle function. Genome-wide DNA methylation of myotube cultures established from middle-aged obese men before and after endurance exercise training identified a differentially methylated region (DMR) located downstream ofGremlin 1(GREM1), which was associated with increasedGREM1expression. GREM1 expression was lower in muscle satellite cells from obese, compared to lean mice, and exercise training restored GREM1 levels to those of control animals. We show that GREM1 regulates muscle differentiation through the negative control of satellite cell self-renewal, and that GREM1 controls muscle lineage commitment and lipid oxidation through the AMPK pathway. Our study identifies novel functions of GREM1 and reveals an epigenetic mechanism by which exercise training reprograms muscle stem cells to improve skeletal muscle function..
| Medienart: |
|
|---|
Preprint| Erscheinungsjahr: |
2022 |
|---|---|
| Erschienen: |
2022 |
| Enthalten in: |
bioRxiv.org - (2022) vom: 16. Okt. Zur Gesamtaufnahme - year:2022 |
|---|
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Fabre, Odile [VerfasserIn] |
|---|
| Links: |
Volltext [kostenfrei] |
|---|
| Themen: |
|---|
| doi: |
10.1101/2020.02.20.956300 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
XBI000741078 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | XBI000741078 | ||
| 003 | DE-627 | ||
| 005 | 20230429091649.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200313s2022 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1101/2020.02.20.956300 |2 doi | |
| 035 | |a (DE-627)XBI000741078 | ||
| 035 | |a (biorXiv)10.1101/2020.02.20.956300 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Fabre, Odile |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a GREM1 is epigenetically reprogrammed in muscle cells after exercise training and controls myogenesis and metabolism |
| 264 | 1 | |c 2022 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a Abstract Exercise training improves skeletal muscle function, notably through tissue regeneration by muscle stem cells. Here, we hypothesized that exercise training reprograms the epigenome of muscle cell, which could account for better muscle function. Genome-wide DNA methylation of myotube cultures established from middle-aged obese men before and after endurance exercise training identified a differentially methylated region (DMR) located downstream ofGremlin 1(GREM1), which was associated with increasedGREM1expression. GREM1 expression was lower in muscle satellite cells from obese, compared to lean mice, and exercise training restored GREM1 levels to those of control animals. We show that GREM1 regulates muscle differentiation through the negative control of satellite cell self-renewal, and that GREM1 controls muscle lineage commitment and lipid oxidation through the AMPK pathway. Our study identifies novel functions of GREM1 and reveals an epigenetic mechanism by which exercise training reprograms muscle stem cells to improve skeletal muscle function. | ||
| 650 | 4 | |a Biology |7 (dpeaa)DE-84 | |
| 650 | 4 | |a 570 |7 (dpeaa)DE-84 | |
| 700 | 1 | |a Giordani, Lorenzo |e verfasserin |4 aut | |
| 700 | 1 | |a Parisi, Alice |e verfasserin |4 aut | |
| 700 | 1 | |a Pattamaprapanont, Pattarawan |e verfasserin |4 aut | |
| 700 | 1 | |a Ahwazi, Danial |e verfasserin |4 aut | |
| 700 | 1 | |a Brun, Caroline |e verfasserin |4 aut | |
| 700 | 1 | |a Chakroun, Imane |e verfasserin |4 aut | |
| 700 | 1 | |a Taleb, Anissa |e verfasserin |4 aut | |
| 700 | 1 | |a Blais, Alexandre |e verfasserin |4 aut | |
| 700 | 1 | |a Andersen, Emil |e verfasserin |4 aut | |
| 700 | 1 | |a Ingerslev, Lars R |e verfasserin |4 aut | |
| 700 | 1 | |a Maire, Pascal |e verfasserin |4 aut | |
| 700 | 1 | |a Rudnicki, Michael |e verfasserin |4 aut | |
| 700 | 1 | |a Laurens, Claire |e verfasserin |4 aut | |
| 700 | 1 | |a Citirikkaya, Kiymet |e verfasserin |4 aut | |
| 700 | 1 | |a Garde, Christian |e verfasserin |4 aut | |
| 700 | 1 | |a Lundell, Leonidas |e verfasserin |4 aut | |
| 700 | 1 | |a Deshmukh, Atul S |e verfasserin |4 aut | |
| 700 | 1 | |a Moro, Cédric |e verfasserin |4 aut | |
| 700 | 1 | |a Bourlier, Virginie |e verfasserin |4 aut | |
| 700 | 1 | |a Mounier, Rémi |e verfasserin |4 aut | |
| 700 | 1 | |a Grand, Fabien Le |e verfasserin |4 aut | |
| 700 | 1 | |a Barrès, Romain |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t bioRxiv.org |g (2022) vom: 16. Okt. |
| 773 | 1 | 8 | |g year:2022 |g day:16 |g month:10 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1101/2020.02.20.956300 |z kostenfrei |3 Volltext |
| 912 | |a GBV_XBI | ||
| 912 | |a SSG-OLC-PHA | ||
| 951 | |a AR | ||
| 952 | |j 2022 |b 16 |c 10 | ||