OBERON3 and SUPPRESSOR OF MAX2 1-LIKE proteins form a regulatory module specifying phloem identity
Abstract Spatial specificity of cell fate decisions is central for organismal development. The phloem tissue mediates long-distance transport of energy metabolites along plant bodies and is characterized by an exceptional degree of cellular specialization. How a phloem-specific developmental program is implemented is, however, unknown. Here we reveal that the ubiquitously expressed PHD-finger protein OBE3 forms a central module with the phloem- specific SMXL5 protein for establishing phloem identity inArabidopsis thaliana. By protein interaction studies and phloem-specific ATAC-seq analyses, we show that OBE3 and SMXL5 proteins form a complex in nuclei of phloem stem cells where they establish a phloem-specific chromatin profile. This profile allows expression ofOPS,BRX,BAM3, andCVP2genes acting as mediators of phloem differentiation. Our findings demonstrate that OBE3/SMXL5 protein complexes establish nuclear features essential for determining phloem cell fate and highlight how a combination of ubiquitous and local regulators generate specificity of developmental decisions in plants..
| Medienart: |
|
|---|
Preprint| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
bioRxiv.org - (2023) vom: 24. Sept. Zur Gesamtaufnahme - year:2023 |
|---|
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Wallner, Eva-Sophie [VerfasserIn] |
|---|
| Links: |
Volltext [kostenfrei] |
|---|
| Themen: |
|---|
| doi: |
10.1101/2019.12.21.885863 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
XBI000686611 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | XBI000686611 | ||
| 003 | DE-627 | ||
| 005 | 20231205150530.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200313s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1101/2019.12.21.885863 |2 doi | |
| 035 | |a (DE-627)XBI000686611 | ||
| 035 | |a (biorXiv)10.1101/2019.12.21.885863 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Wallner, Eva-Sophie |e verfasserin |0 (orcid)0000-0002-4268-3976 |4 aut | |
| 245 | 1 | 0 | |a OBERON3 and SUPPRESSOR OF MAX2 1-LIKE proteins form a regulatory module specifying phloem identity |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a Abstract Spatial specificity of cell fate decisions is central for organismal development. The phloem tissue mediates long-distance transport of energy metabolites along plant bodies and is characterized by an exceptional degree of cellular specialization. How a phloem-specific developmental program is implemented is, however, unknown. Here we reveal that the ubiquitously expressed PHD-finger protein OBE3 forms a central module with the phloem- specific SMXL5 protein for establishing phloem identity inArabidopsis thaliana. By protein interaction studies and phloem-specific ATAC-seq analyses, we show that OBE3 and SMXL5 proteins form a complex in nuclei of phloem stem cells where they establish a phloem-specific chromatin profile. This profile allows expression ofOPS,BRX,BAM3, andCVP2genes acting as mediators of phloem differentiation. Our findings demonstrate that OBE3/SMXL5 protein complexes establish nuclear features essential for determining phloem cell fate and highlight how a combination of ubiquitous and local regulators generate specificity of developmental decisions in plants. | ||
| 650 | 4 | |a Biology |7 (dpeaa)DE-84 | |
| 650 | 4 | |a 570 |7 (dpeaa)DE-84 | |
| 700 | 1 | |a Tonn, Nina |4 aut | |
| 700 | 1 | |a Shi, Dongbo |0 (orcid)0000-0002-4408-3042 |4 aut | |
| 700 | 1 | |a Luzzietti, Laura |4 aut | |
| 700 | 1 | |a Wanke, Friederike |0 (orcid)0000-0001-6080-8800 |4 aut | |
| 700 | 1 | |a Hunziker, Pascal |0 (orcid)0000-0002-4589-3047 |4 aut | |
| 700 | 1 | |a Xu, Yingqiang |4 aut | |
| 700 | 1 | |a Jung, Ilona |4 aut | |
| 700 | 1 | |a Lopéz-Salmerón, Vadir |4 aut | |
| 700 | 1 | |a Gebert, Michael |4 aut | |
| 700 | 1 | |a Wenzl, Christian |4 aut | |
| 700 | 1 | |a Lohmann, Jan U. |0 (orcid)0000-0003-3667-187X |4 aut | |
| 700 | 1 | |a Harter, Klaus |0 (orcid)0000-0002-2150-6970 |4 aut | |
| 700 | 1 | |a Greb, Thomas |0 (orcid)0000-0002-6176-646X |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t bioRxiv.org |g (2023) vom: 24. Sept. |
| 773 | 1 | 8 | |g year:2023 |g day:24 |g month:09 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1101/2019.12.21.885863 |z kostenfrei |3 Volltext |
| 912 | |a GBV_XBI | ||
| 951 | |a AR | ||
| 952 | |j 2023 |b 24 |c 09 | ||