Kilohertz two-photon fluorescence microscopy imaging of neural activity<i>in vivo</i>
Understanding information processing in the brain requires us to monitor neural activityin vivoat high spatiotemporal resolution. Using an ultrafast two-photon fluorescence microscope (2PFM) empowered by all-optical laser scanning, we imaged neural activityin vivoat up to 3,000 frames per second and submicron spatial resolution. This ultrafast imaging method enabled monitoring of both supra- and sub-threshold electrical activity down to 345 μm below the brain surface in head fixed awake mice..
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Preprint| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
bioRxiv.org - (2023) vom: 13. Sept. Zur Gesamtaufnahme - year:2023 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wu, Jianglai [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| Themen: |
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| doi: |
10.1101/543058 |
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| PPN (Katalog-ID): |
XBI000447501 |
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| 520 | |a Understanding information processing in the brain requires us to monitor neural activityin vivoat high spatiotemporal resolution. Using an ultrafast two-photon fluorescence microscope (2PFM) empowered by all-optical laser scanning, we imaged neural activityin vivoat up to 3,000 frames per second and submicron spatial resolution. This ultrafast imaging method enabled monitoring of both supra- and sub-threshold electrical activity down to 345 μm below the brain surface in head fixed awake mice. | ||
| 650 | 4 | |a Biology |7 (dpeaa)DE-84 | |
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| 700 | 1 | |a Liang, Yajie |4 aut | |
| 700 | 1 | |a Chen, Shuo |4 aut | |
| 700 | 1 | |a Hsu, Ching-Lung |4 aut | |
| 700 | 1 | |a Chavarha, Mariya |4 aut | |
| 700 | 1 | |a Evans, Stephen W |4 aut | |
| 700 | 1 | |a Shi, Donqging |4 aut | |
| 700 | 1 | |a Lin, Michael Z |4 aut | |
| 700 | 1 | |a Tsia, Kevin K |0 (orcid)0000-0002-6394-9657 |4 aut | |
| 700 | 1 | |a Ji, Na |4 aut | |
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