Silencing of V‐ATPase‐E gene causes midgut apoptosis of Diaphorina citri and affects its acquisition of Huanglongbing pathogen
Abstract The Asian citrus psyllid, Diaphorina citri Kuwayama, is among the most important pests of citrus. It is the main vector of the Huanglongbing (HLB) pathogen Candidatus Liberibacter asiaticus ( C Las), which causes severe losses in citrus crops. Control of D. citri is therefore of paramount importance to reduce the spread of HLB. In this regard, using RNA interference (RNAi) to silence target genes is a useful strategy to control psyllids. In this study, using RNAi, we examined the biological functions of the V‐ATPase subunit E( V‐ATP‐E) gene of D. citri, including its effect on acquisition of C Las. The amino acid sequence of V‐ATP‐E from D. citri had high homology with proteins from other insects. V‐ATP‐E was expressed at all D. citri life stages analyzed, and the expression level in mature adults was higher than that of teneral adults. Silencing of V‐ATP‐E resulted in a significant increase in mortality, reduced body weight, and induced cell apoptosis of the D. citri midgut. The reduced expression of V‐ATP‐E was indicated to inhibit C Las passing through the midgut and into the hemolymph, leading to a majority of C Las being confined to the midgut. In addition, double‐stranded RNA of D. citri V‐ATP‐E was safe to non‐target parasitic wasps. These results suggest that V‐ATP‐E is an effective RNAi target that can be used in D. citri control to block C Las infection..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:30 |
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| Enthalten in: |
Insect Science - 30(2023), 4, Seite 1022-1034 |
| Beteiligte Personen: |
Guo, Chang‐Fei [VerfasserIn] |
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| Anmerkungen: |
© 2023 Institute of Zoology, Chinese Academy of Sciences. |
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| Umfang: |
13 |
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| doi: |
10.1111/1744-7917.13146 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
WLY015996778 |
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| 245 | 1 | 0 | |a Silencing of V‐ATPase‐E gene causes midgut apoptosis of Diaphorina citri and affects its acquisition of Huanglongbing pathogen |
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| 520 | |a Abstract The Asian citrus psyllid, Diaphorina citri Kuwayama, is among the most important pests of citrus. It is the main vector of the Huanglongbing (HLB) pathogen Candidatus Liberibacter asiaticus ( C Las), which causes severe losses in citrus crops. Control of D. citri is therefore of paramount importance to reduce the spread of HLB. In this regard, using RNA interference (RNAi) to silence target genes is a useful strategy to control psyllids. In this study, using RNAi, we examined the biological functions of the V‐ATPase subunit E( V‐ATP‐E) gene of D. citri, including its effect on acquisition of C Las. The amino acid sequence of V‐ATP‐E from D. citri had high homology with proteins from other insects. V‐ATP‐E was expressed at all D. citri life stages analyzed, and the expression level in mature adults was higher than that of teneral adults. Silencing of V‐ATP‐E resulted in a significant increase in mortality, reduced body weight, and induced cell apoptosis of the D. citri midgut. The reduced expression of V‐ATP‐E was indicated to inhibit C Las passing through the midgut and into the hemolymph, leading to a majority of C Las being confined to the midgut. In addition, double‐stranded RNA of D. citri V‐ATP‐E was safe to non‐target parasitic wasps. These results suggest that V‐ATP‐E is an effective RNAi target that can be used in D. citri control to block C Las infection. | ||
| 700 | 1 | |a Qiu, Jun‐Hong |4 aut | |
| 700 | 1 | |a Hu, Yu‐Wei |4 aut | |
| 700 | 1 | |a Xu, Pei‐Ping |4 aut | |
| 700 | 1 | |a Deng, Ying‐Qi |4 aut | |
| 700 | 1 | |a Tian, Ling |4 aut | |
| 700 | 1 | |a Wei, Yi‐Yun |4 aut | |
| 700 | 1 | |a Sang, Wen |4 aut | |
| 700 | 1 | |a Liu, Yu‐Tao |4 aut | |
| 700 | 1 | |a Qiu, Bao‐Li |4 aut | |
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