Nursery outbreak caused by enteroaggregative Escherichia coli serogroup O111 : H21
This study describes, for the first time, the occurrence of an epidemic of enteroaggregative Escherichia coli(EAEC) O111:H21 in a Belgian nursery and describes a practical approach concerning its management. Few data exist in the literature on this type of outbreak. Clinical and microbiological investigations were needed to find a link between the cases and identify the causative agent. The microbiological procedure followed was first based on conventional analyses: isolation using selective cultures, identification by MALDI‐TOF MS, antibiogram, determination of the serogroup by agglutination, then whole genome sequencing. A total of 7/21 children were infected with this pathogen. Four cases could be confirmed by a molecular technique, wgMLST, as belonging to the same bacterial clone. The action plan put in place focused on symptomatic case eviction, strict general hygiene precaution as well as specific cleaning and disinfection measures. The epidemic did last only a few days. It appears important, in the context of an epidemic, that clinical laboratories standardize their practice by equipping themselves with molecular techniques such as a multiplex which does not focus only on the serotype O157:H7 and which make it possible to distinguish the different pathotypes of E. coli by targeting several virulence genes ( stx, agg R…). However, cost/effectiveness studies are awaited to confirm the interest of a systematic search by molecular method for the pathogen involved in a suspected outbreak occurring in a nursery..
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:131 |
|---|---|
| Enthalten in: |
APMIS - 131(2023), 5, Seite 206-216 |
| Beteiligte Personen: |
Tré‐Hardy, Marie [VerfasserIn] |
|---|
| Anmerkungen: |
© 2023 Scandinavian Societies for Pathology, Medical Microbiology and Immunology. |
|---|
| Umfang: |
216 |
|---|
| doi: |
10.1111/apm.13301 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
WLY015077500 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | WLY015077500 | ||
| 003 | DE-627 | ||
| 005 | 20231111130026.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231111s2023 xx |||||o 00| ||und c | ||
| 024 | 7 | |a 10.1111/apm.13301 |2 doi | |
| 028 | 5 | 2 | |a 2023-11-11_APM_2023_131_5.zip.xml |
| 035 | |a (DE-627)WLY015077500 | ||
| 035 | |a (WILEY)APM13301 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 100 | 1 | |a Tré‐Hardy, Marie |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Nursery outbreak caused by enteroaggregative Escherichia coli serogroup O111 |b H21 |
| 264 | 1 | |c 2023 | |
| 300 | |a 216 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a © 2023 Scandinavian Societies for Pathology, Medical Microbiology and Immunology. | ||
| 520 | |a This study describes, for the first time, the occurrence of an epidemic of enteroaggregative Escherichia coli(EAEC) O111:H21 in a Belgian nursery and describes a practical approach concerning its management. Few data exist in the literature on this type of outbreak. Clinical and microbiological investigations were needed to find a link between the cases and identify the causative agent. The microbiological procedure followed was first based on conventional analyses: isolation using selective cultures, identification by MALDI‐TOF MS, antibiogram, determination of the serogroup by agglutination, then whole genome sequencing. A total of 7/21 children were infected with this pathogen. Four cases could be confirmed by a molecular technique, wgMLST, as belonging to the same bacterial clone. The action plan put in place focused on symptomatic case eviction, strict general hygiene precaution as well as specific cleaning and disinfection measures. The epidemic did last only a few days. It appears important, in the context of an epidemic, that clinical laboratories standardize their practice by equipping themselves with molecular techniques such as a multiplex which does not focus only on the serotype O157:H7 and which make it possible to distinguish the different pathotypes of E. coli by targeting several virulence genes ( stx, agg R…). However, cost/effectiveness studies are awaited to confirm the interest of a systematic search by molecular method for the pathogen involved in a suspected outbreak occurring in a nursery. | ||
| 700 | 1 | |a De Rauw, Klara |4 aut | |
| 700 | 1 | |a Blairon, Laurent |4 aut | |
| 700 | 1 | |a Simon, Anne |4 aut | |
| 700 | 1 | |a Piérard, Denis |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t APMIS |g 131(2023), 5, Seite 206-216 |w (DE-627)WLY002253992 |x 16000463 |7 nnns |
| 773 | 1 | 8 | |g volume:131 |g year:2023 |g number:5 |g pages:206-216 |g extent:216 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_WLY | ||
| 951 | |a AR | ||
| 952 | |d 131 |j 2023 |e 5 |h 206-216 |g 216 | ||