Development of Dissolution Dynamic Nuclear Polarization of [15 N3]Metronidazole : A Clinically Approved Antibiotic
Abstract We report dissolution Dynamic Nuclear Polarization (d‐DNP) of [15 N3]metronidazole ([15 N3]MNZ) for the first time. Metronidazole is a clinically approved antibiotic, which can be potentially employed as a hypoxia‐sensing molecular probe using15 N hyperpolarized (HP) nucleus. The DNP process is very efficient for [15 N3]MNZ with an exponential build‐up constant of 13.8 min using trityl radical. After dissolution and sample transfer to a nearby 4.7 T Magnetic Resonance Imaging scanner, HP [15 N3]MNZ lasted remarkably long with T1 values up to 343 s and15 N polarizations up to 6.4 %. A time series of HP [15 N3]MNZ images was acquired in vitro using a steady state free precession sequence on the15 NO2 peak. The signal lasted over 13 min with notably long T2 of 20.5 s. HP [15 N3]MNZ was injected in the tail vein of a healthy rat, and dynamic spectroscopy was performed over the rat brain. The in vivo HP15 N signals persisted over 70 s, demonstrating an unprecedented opportunity for in vivo studies..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:62 |
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| Enthalten in: |
Angewandte Chemie International Edition - 62(2023), 31 |
| Beteiligte Personen: |
Guarin, David O. [VerfasserIn] |
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| Anmerkungen: |
© 2023 Wiley‐VCH GmbH |
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| Umfang: |
6 |
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| doi: |
10.1002/anie.202219181 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 100 | 1 | |a Guarin, David O. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Development of Dissolution Dynamic Nuclear Polarization of [15 N3]Metronidazole |b A Clinically Approved Antibiotic |
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| 520 | |a Abstract We report dissolution Dynamic Nuclear Polarization (d‐DNP) of [15 N3]metronidazole ([15 N3]MNZ) for the first time. Metronidazole is a clinically approved antibiotic, which can be potentially employed as a hypoxia‐sensing molecular probe using15 N hyperpolarized (HP) nucleus. The DNP process is very efficient for [15 N3]MNZ with an exponential build‐up constant of 13.8 min using trityl radical. After dissolution and sample transfer to a nearby 4.7 T Magnetic Resonance Imaging scanner, HP [15 N3]MNZ lasted remarkably long with T1 values up to 343 s and15 N polarizations up to 6.4 %. A time series of HP [15 N3]MNZ images was acquired in vitro using a steady state free precession sequence on the15 NO2 peak. The signal lasted over 13 min with notably long T2 of 20.5 s. HP [15 N3]MNZ was injected in the tail vein of a healthy rat, and dynamic spectroscopy was performed over the rat brain. The in vivo HP15 N signals persisted over 70 s, demonstrating an unprecedented opportunity for in vivo studies. | ||
| 700 | 1 | |a Joshi, Sameer M. |4 aut | |
| 700 | 1 | |a Samoilenko, Anna |4 aut | |
| 700 | 1 | |a Kabir, Mohammad S. H. |4 aut | |
| 700 | 1 | |a Hardy, Erin E. |4 aut | |
| 700 | 1 | |a Takahashi, Atsush M. |4 aut | |
| 700 | 1 | |a Ardenkjaer‐Larsen, Jan H. |4 aut | |
| 700 | 1 | |a Chekmenev, Eduard Y. |4 aut | |
| 700 | 1 | |a Yen, Yi‐Fen |4 aut | |
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