In vitro Studies of Antitumor Effect, Toxicity/Cytotoxicity and Skin Permeation/Retention of a Green Fluorescence Pyrene‐based Dye for PDT Application
Abstract Photosensitizers (PS) are compounds that can generate reactive oxygen species under irradiation of appropriate light and are widely used in photodynamic therapy (PDT). Currently, topical PDT is an effective treatment for several skin diseases, including bacterial infections, fungal mycoses and psoriasis. In addition, PDT is also used to treat nonmelanoma skin cancer and can be a potential tool for melanoma, associated with other treatments. In this work, we evaluated the antitumor photoactivity of a new pyrene‐based PS (TPPy) by using the murine melanoma cell line (B16F10). The in vitro permeation/retention tests in porcine ear skin were also performed in order to evaluate the potential application of the PS for topical use in skin cancer. Moreover, to determine the toxicity in vivo, we used the Galleria mellonella as an alternative animal model of study. The results showed that TPPy is a promising PS for application in PDT, with potential antitumor photoactivity (IC506.5 μmol L‐1), absence of toxicity in the G. mellonella model at higher concentration (70.0 mmol L−1) and the accumulation tendency in the epidermis plus dermis sites (165.20 ± 4.12 ng cm−2)..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:97 |
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| Enthalten in: |
Photochemistry and Photobiology - 97(2021), 2, Seite 408-415 |
| Beteiligte Personen: |
de França, Bruna Martins [VerfasserIn] |
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| BKL: |
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| Anmerkungen: |
Copyright © 2021 American Society for Photobiology |
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| Umfang: |
8 |
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| doi: |
10.1111/php.13335 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Abstract Photosensitizers (PS) are compounds that can generate reactive oxygen species under irradiation of appropriate light and are widely used in photodynamic therapy (PDT). Currently, topical PDT is an effective treatment for several skin diseases, including bacterial infections, fungal mycoses and psoriasis. In addition, PDT is also used to treat nonmelanoma skin cancer and can be a potential tool for melanoma, associated with other treatments. In this work, we evaluated the antitumor photoactivity of a new pyrene‐based PS (TPPy) by using the murine melanoma cell line (B16F10). The in vitro permeation/retention tests in porcine ear skin were also performed in order to evaluate the potential application of the PS for topical use in skin cancer. Moreover, to determine the toxicity in vivo, we used the Galleria mellonella as an alternative animal model of study. The results showed that TPPy is a promising PS for application in PDT, with potential antitumor photoactivity (IC506.5 μmol L‐1), absence of toxicity in the G. mellonella model at higher concentration (70.0 mmol L−1) and the accumulation tendency in the epidermis plus dermis sites (165.20 ± 4.12 ng cm−2). | ||
| 700 | 1 | |a Ghasemishahrestani, Zeinab |4 aut | |
| 700 | 1 | |a de Souza, Gabriel Ferreira Motta |4 aut | |
| 700 | 1 | |a da Silva, Renata Nascimento |4 aut | |
| 700 | 1 | |a Queiroz, Daniela Dias |4 aut | |
| 700 | 1 | |a Pierre, Maria Bernadete Riemma |4 aut | |
| 700 | 1 | |a Pereira, Marcos Dias |4 aut | |
| 700 | 1 | |a Forero, Josué Sebastián Bello |4 aut | |
| 700 | 1 | |a Corrêa, Rodrigo José |4 aut | |
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