Laser‐enabled delivery of antiretroviral drugs into HIV‐1 infected TZM‐bl cells
Abstract The use of femtosecond laser to create sub‐microscopic transient pores on the cell membrane allowing exogenous material into mammalian cells has become a very efficient optical delivery method over the past decade. This study focuses on laser‐enabled delivery of antiretroviral (ARV) drugs into HIV‐1 infected TZM‐bl cells in vitro. A 1 kHz femtosecond laser emitting at a wavelength of 800 nm was used to photoporate cells at 6.5 μW. Trypan blue was used for characterisation and its uptake was quantified using Matlab software. Cell membrane damage was assessed using the lactate dehydrogenase (LDH) assay while HIV‐1 infection was assessed using luciferase assay. Our results showed successful delivery of ARVs into HIV‐1 infected cells without compromising their cell membranes, subsequently reducing the level of infection. The LDH assay showed no significant cell membrane damage of laser‐treated cells, and the luciferase assay demonstrated significant reduction in the level of HIV‐1 infection..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:15 |
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| Enthalten in: |
Journal of Biophotonics - 15(2022), 10 |
| Beteiligte Personen: |
Maphanga, Charles [VerfasserIn] |
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| BKL: |
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| Anmerkungen: |
© 2022 Wiley‐VCH GmbH |
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| Umfang: |
11 |
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| doi: |
10.1002/jbio.202200043 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Abstract The use of femtosecond laser to create sub‐microscopic transient pores on the cell membrane allowing exogenous material into mammalian cells has become a very efficient optical delivery method over the past decade. This study focuses on laser‐enabled delivery of antiretroviral (ARV) drugs into HIV‐1 infected TZM‐bl cells in vitro. A 1 kHz femtosecond laser emitting at a wavelength of 800 nm was used to photoporate cells at 6.5 μW. Trypan blue was used for characterisation and its uptake was quantified using Matlab software. Cell membrane damage was assessed using the lactate dehydrogenase (LDH) assay while HIV‐1 infection was assessed using luciferase assay. Our results showed successful delivery of ARVs into HIV‐1 infected cells without compromising their cell membranes, subsequently reducing the level of infection. The LDH assay showed no significant cell membrane damage of laser‐treated cells, and the luciferase assay demonstrated significant reduction in the level of HIV‐1 infection. | ||
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