In situ Quantification of Cellular Cholesterol using a Molecular Sensor
Cholesterol has been implicated in regulation of physical properties of the plasma membrane, but its direct involvement in cell regulation through specific interactions with cellular proteins has not been thoroughly investigated. Also, the quantitative information about its cellular spatiotemporal dynamics and its local concentration fluctuation under pathophysiological conditions is lacking. To apply our recently developed quantitative lipid imaging technology to in situ quantification of cholesterol, we prepared a panel of cholesterol‐specific sensors from various cholesterol binding proteins that have different dynamic ranges of cholesterol detection. In situ quantification of cholesterol in various mammalian cells using these sensors revealed new insight into the spatiotemporal dynamics and local fluctuation of cholesterol under various physiological conditions..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2013 |
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| Erschienen: |
2013 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:27 |
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| Enthalten in: |
The FASEB Journal - 27(2013), Seite lb89-lb89 |
| Beteiligte Personen: |
Stec, Ewa [VerfasserIn] |
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| BKL: |
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| Anmerkungen: |
© Federation of American Societies for Experimental Biology |
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| Umfang: |
1 |
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| doi: |
10.1096/fasebj.27.1_supplement.lb89 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Cholesterol has been implicated in regulation of physical properties of the plasma membrane, but its direct involvement in cell regulation through specific interactions with cellular proteins has not been thoroughly investigated. Also, the quantitative information about its cellular spatiotemporal dynamics and its local concentration fluctuation under pathophysiological conditions is lacking. To apply our recently developed quantitative lipid imaging technology to in situ quantification of cholesterol, we prepared a panel of cholesterol‐specific sensors from various cholesterol binding proteins that have different dynamic ranges of cholesterol detection. In situ quantification of cholesterol in various mammalian cells using these sensors revealed new insight into the spatiotemporal dynamics and local fluctuation of cholesterol under various physiological conditions. | ||
| 700 | 1 | |a Afsari, Hamid Samareh |4 aut | |
| 700 | 1 | |a Kurilova, Svetlana |4 aut | |
| 700 | 1 | |a Cho, Wonhwa |4 aut | |
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