Superoxide anion mediates Ang II‐induced ET‐1 expression in mouse adventitial fibroblasts
Our objective was to determine if superoxide anion mediates endothelin‐1 (ET‐1) expression in adventitial fibroblasts in response to angiotensin II (Ang II). Vascular adventitial fibroblasts were isolated from mouse aorta, preproET‐1 messenger RNA (mRNA) levels were determined by relative RT‐PCR and procollagen protein was measured by Western blot. Adventitial fibroblasts were treated with or without Ang II (10−7 M) and antioxidants superoxide dismutase (SOD) (350 U/ml), tempol (10−5 M), and tiron (10−5 M). Ang II significantly increased preproET‐1 mRNA and procollagen expression while treatment with antioxidants decreased both significantly (p<0.05). To further examine the role of superoxide, Cu2+/Zn2+ SOD was overexpressed in the adventitial fibroblasts and decreased levels of preproET‐1 mRNA and procollagen were found with Ang II treatment when compared to control (p<0.05). In conclusion, Ang II is able to increase both superoxide and ET‐1 production and inhibition of superoxide leads to a decrease in ET‐1 expression demonstrating that superoxide mediates Ang II induced ET‐1 expression in mice adventitial fibroblasts. Funding provided by the Canadian Institute of Health Research..
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2007 |
|---|---|
| Erschienen: |
2007 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:21 |
|---|---|
| Enthalten in: |
The FASEB Journal - 21(2007), 5, Seite A450-A450 |
| Beteiligte Personen: |
Chapman, Sandy [VerfasserIn] |
|---|
| BKL: |
|---|
| Anmerkungen: |
© Federation of American Societies for Experimental Biology |
|---|
| Umfang: |
1 |
|---|
| doi: |
10.1096/fasebj.21.5.A450-c |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
WLY005924774 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | WLY005924774 | ||
| 003 | DE-627 | ||
| 005 | 20230307154339.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230215s2007 xx |||||o 00| ||und c | ||
| 024 | 7 | |a 10.1096/fasebj.21.5.A450-c |2 doi | |
| 028 | 5 | 2 | |a FSB2_FSB2FASEBJ215A450C.xml |
| 035 | |a (DE-627)WLY005924774 | ||
| 035 | |a (WILEY)FSB2FASEBJ215A450C | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 082 | 0 | 4 | |a 570 |q ASE |
| 084 | |a 42.00 |2 bkl | ||
| 100 | 1 | |a Chapman, Sandy |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Superoxide anion mediates Ang II‐induced ET‐1 expression in mouse adventitial fibroblasts |
| 264 | 1 | |c 2007 | |
| 300 | |a 1 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a © Federation of American Societies for Experimental Biology | ||
| 520 | |a Our objective was to determine if superoxide anion mediates endothelin‐1 (ET‐1) expression in adventitial fibroblasts in response to angiotensin II (Ang II). Vascular adventitial fibroblasts were isolated from mouse aorta, preproET‐1 messenger RNA (mRNA) levels were determined by relative RT‐PCR and procollagen protein was measured by Western blot. Adventitial fibroblasts were treated with or without Ang II (10−7 M) and antioxidants superoxide dismutase (SOD) (350 U/ml), tempol (10−5 M), and tiron (10−5 M). Ang II significantly increased preproET‐1 mRNA and procollagen expression while treatment with antioxidants decreased both significantly (p<0.05). To further examine the role of superoxide, Cu2+/Zn2+ SOD was overexpressed in the adventitial fibroblasts and decreased levels of preproET‐1 mRNA and procollagen were found with Ang II treatment when compared to control (p<0.05). In conclusion, Ang II is able to increase both superoxide and ET‐1 production and inhibition of superoxide leads to a decrease in ET‐1 expression demonstrating that superoxide mediates Ang II induced ET‐1 expression in mice adventitial fibroblasts. Funding provided by the Canadian Institute of Health Research. | ||
| 700 | 1 | |a An, Shengjun |4 aut | |
| 700 | 1 | |a Di Wang, Hui |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t The FASEB Journal |g 21(2007), 5, Seite A450-A450 |w (DE-627)WLY005785006 |x 15306860 |7 nnns |
| 773 | 1 | 8 | |g volume:21 |g year:2007 |g number:5 |g pages:A450-A450 |g extent:1 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_WLY | ||
| 936 | b | k | |a 42.00 |q ASE |
| 951 | |a AR | ||
| 952 | |d 21 |j 2007 |e 5 |h A450-A450 |g 1 | ||