Role of NADPH oxidase in angiotensin II induced endothelin‐1 expression within vascular adventitial fibroblasts
Recent studies have demonstrated that under Angiotensin II (Ang II) treatment, adventitial fibroblasts are able to express Endothelin‐1 (ET‐1), a result which led to increased collagen I expression. However, mediation of the Ang II induced ET‐1 expression through NADPH oxidase in adventitial fibroblasts remains uncertain. The purpose of the present study was to determine if NADPH oxidase in adventitial fibroblasts mediates Ang II induced ET‐1 expression. Vascular adventitial fibroblasts were isolated mouse aorta and treated with Ang II (10‐7 M) in the presence or absence of apocynin (10‐5M), a specific NADPH oxidase inhibitor. PreporET‐1 mRNA was determined by relative RT‐PCR. ET‐1 peptide levels in medium were determined via ELISA. Ang II (10‐7 M) treatment significantly increased both preproET‐1 mRNA and ET‐1 peptide levels (p<0.05), which were significantly decreased by DPI (10‐5M). In addition, the Ang II induced ET‐1 expressions were decreased in cells isolated from the gp91 knock out mice, which lacked a key subunit of NADPH oxidase, therefore decreasing functionality. In conclusion, ET‐1 mRNA and peptide levels are increased after Ang II treatment. Moreover, NADPH oxidase, an important mediator of reactive oxygen species does seem to play an important role in the Ang II induced ET‐1 expression in adventitial fibroblasts..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2007 |
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| Erschienen: |
2007 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:21 |
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| Enthalten in: |
The FASEB Journal - 21(2007), 5, Seite A450-A450 |
| Beteiligte Personen: |
Boyd, Ryan Cameron [VerfasserIn] |
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| BKL: |
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| Anmerkungen: |
© Federation of American Societies for Experimental Biology |
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| Umfang: |
1 |
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| doi: |
10.1096/fasebj.21.5.A450 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Recent studies have demonstrated that under Angiotensin II (Ang II) treatment, adventitial fibroblasts are able to express Endothelin‐1 (ET‐1), a result which led to increased collagen I expression. However, mediation of the Ang II induced ET‐1 expression through NADPH oxidase in adventitial fibroblasts remains uncertain. The purpose of the present study was to determine if NADPH oxidase in adventitial fibroblasts mediates Ang II induced ET‐1 expression. Vascular adventitial fibroblasts were isolated mouse aorta and treated with Ang II (10‐7 M) in the presence or absence of apocynin (10‐5M), a specific NADPH oxidase inhibitor. PreporET‐1 mRNA was determined by relative RT‐PCR. ET‐1 peptide levels in medium were determined via ELISA. Ang II (10‐7 M) treatment significantly increased both preproET‐1 mRNA and ET‐1 peptide levels (p<0.05), which were significantly decreased by DPI (10‐5M). In addition, the Ang II induced ET‐1 expressions were decreased in cells isolated from the gp91 knock out mice, which lacked a key subunit of NADPH oxidase, therefore decreasing functionality. In conclusion, ET‐1 mRNA and peptide levels are increased after Ang II treatment. Moreover, NADPH oxidase, an important mediator of reactive oxygen species does seem to play an important role in the Ang II induced ET‐1 expression in adventitial fibroblasts. | ||
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