Silencing P38 MAPK reduces cellular senescence in human fetal chorion trophoblast cells
Abstract Problem Amniochorion senescence generates mechanistic signals to initiate parturition. Activation of p38 mitogen‐activated kinase (MAPK) in fetal amnion cells is a key mediator of senescence as well as epithelial‐mesenchymal transition (EMT) of amnion cells. However, the impact of p38 MAPK in chorion trophoblast cells (CTCs) is unclear. We tested if eliminating p38 will reduce oxidative stress (OS) induced cell fates like cellular senescence, EMT, and inflammation induced by these processes in CTCs. Methods p38MAPK in CTCs was silenced using CRISPR/Cas9. OS was evoked by cigarette smoke extract (CSE) exposure. EMT was evoked by transforming growth factor (TGF)‐ß treatment. Cell cycle, senescence, EMT, and inflammation were analyzed. Results CSE‐induced changes in the cell cycle were not seen in p38KO CTCs compared to WT cells. OS induced by CSE evoked senescence and senescence‐associated secretory phenotype (SASP as indicated by IL‐6 and IL‐8 increase) in WT but not in p38MAPK KO CTCs. No changes were noted in HLA‐G expression regardless of the status of p38MAPK. Neither CSE nor TGF‐ß evoked EMT in either WT or p38 KO CTCs. Conclusion Senescence and senescence‐associated inflammation in human fetal CTCs are mediated by p38MAPK. Compared to amnion epithelial cells, CTCs are resistant to EMT. This refractoriness may help them to maintain the barrier functions at the choriodecidual interface..
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:89 |
|---|---|
| Enthalten in: |
American Journal of Reproductive Immunology - 89(2023), 1 |
| Beteiligte Personen: |
Goldman, Brett [VerfasserIn] |
|---|
| BKL: |
|---|
| Anmerkungen: |
© 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. |
|---|
| Umfang: |
10 |
|---|
| doi: |
10.1111/aji.13648 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
WLY001140434 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | WLY001140434 | ||
| 003 | DE-627 | ||
| 005 | 20230307101240.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230210s2023 xx |||||o 00| ||und c | ||
| 024 | 7 | |a 10.1111/aji.13648 |2 doi | |
| 028 | 5 | 2 | |a AJI_AJI13648.xml |
| 035 | |a (DE-627)WLY001140434 | ||
| 035 | |a (WILEY)AJI13648 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 082 | 0 | 4 | |a 610 |q ASE |
| 084 | |a 44.45 |2 bkl | ||
| 084 | |a 44.74 |2 bkl | ||
| 100 | 1 | |a Goldman, Brett |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Silencing P38 MAPK reduces cellular senescence in human fetal chorion trophoblast cells |
| 264 | 1 | |c 2023 | |
| 300 | |a 10 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a © 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. | ||
| 520 | |a Abstract Problem Amniochorion senescence generates mechanistic signals to initiate parturition. Activation of p38 mitogen‐activated kinase (MAPK) in fetal amnion cells is a key mediator of senescence as well as epithelial‐mesenchymal transition (EMT) of amnion cells. However, the impact of p38 MAPK in chorion trophoblast cells (CTCs) is unclear. We tested if eliminating p38 will reduce oxidative stress (OS) induced cell fates like cellular senescence, EMT, and inflammation induced by these processes in CTCs. Methods p38MAPK in CTCs was silenced using CRISPR/Cas9. OS was evoked by cigarette smoke extract (CSE) exposure. EMT was evoked by transforming growth factor (TGF)‐ß treatment. Cell cycle, senescence, EMT, and inflammation were analyzed. Results CSE‐induced changes in the cell cycle were not seen in p38KO CTCs compared to WT cells. OS induced by CSE evoked senescence and senescence‐associated secretory phenotype (SASP as indicated by IL‐6 and IL‐8 increase) in WT but not in p38MAPK KO CTCs. No changes were noted in HLA‐G expression regardless of the status of p38MAPK. Neither CSE nor TGF‐ß evoked EMT in either WT or p38 KO CTCs. Conclusion Senescence and senescence‐associated inflammation in human fetal CTCs are mediated by p38MAPK. Compared to amnion epithelial cells, CTCs are resistant to EMT. This refractoriness may help them to maintain the barrier functions at the choriodecidual interface. | ||
| 700 | 1 | |a Radnaa, Enkhtuya |4 aut | |
| 700 | 1 | |a Kechichian, Talar |4 aut | |
| 700 | 1 | |a Menon, Ramkumar |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t American Journal of Reproductive Immunology |g 89(2023), 1 |w (DE-627)WLY001130560 |x 16000897 |7 nnns |
| 773 | 1 | 8 | |g volume:89 |g year:2023 |g number:1 |g extent:10 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_WLY | ||
| 936 | b | k | |a 44.45 |q ASE |
| 936 | b | k | |a 44.74 |q ASE |
| 951 | |a AR | ||
| 952 | |d 89 |j 2023 |e 1 |g 10 | ||