A tempo-spatial controllable microfluidic shear-stress generator for in-vitro mimicking of the thrombus
Pathological conditions linked to shear stress have been identified in hematological diseases, cardiovascular diseases, and cancer. These conditions often exhibit significantly elevated shear stress levels, surpassing 1000 dyn/$ cm^{2} $ in severely stenotic arteries. Heightened shear stress can induce mechanical harm to endothelial cells, potentially leading to bleeding and fatal consequences. However, current technology still grapples with limitations, including inadequate flexibility in simulating bodily shear stress environments, limited range of shear stress generation, and spatial and temporal adaptability. Consequently, a comprehensive understanding of the mechanisms underlying the impact of shear stress on physiological and pathological conditions, like thrombosis, remains inadequate. To address these limitations, this study presents a microfluidic-based shear stress generation chip as a proposed solution. The chip achieves a substantial 929-fold variation in shear stress solely by adjusting the degree of constriction in branch channels after PDMS fabrication. Experiments demonstrated that a rapid increase in shear stress up to 1000 dyn/$ cm^{2} $ significantly detached 88.2% cells from the substrate. Long-term exposure (24 h) to shear stress levels below 8.3 dyn/$ cm^{2} $ did not significantly impact cell growth. Furthermore, cells exposed to shear stress levels equal to or greater than 8.3 dyn/$ cm^{2} $ exhibited significant alterations in aspect ratio and orientation, following a normal distribution. This microfluidic chip provides a reliable tool for investigating cellular responses to the wide-ranging shear stress existing in both physiological and pathological flow conditions. Graphical Abstract.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:22 |
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| Enthalten in: |
Journal of nanobiotechnology - 22(2024), 1 vom: 17. Apr. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yu, Zhihang [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| BKL: | |
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| Themen: |
Biotechnology Microfluidics |
| Anmerkungen: |
© The Author(s) 2024 |
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| doi: |
10.1186/s12951-024-02334-6 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
SPR055561403 |
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| 520 | |a Pathological conditions linked to shear stress have been identified in hematological diseases, cardiovascular diseases, and cancer. These conditions often exhibit significantly elevated shear stress levels, surpassing 1000 dyn/$ cm^{2} $ in severely stenotic arteries. Heightened shear stress can induce mechanical harm to endothelial cells, potentially leading to bleeding and fatal consequences. However, current technology still grapples with limitations, including inadequate flexibility in simulating bodily shear stress environments, limited range of shear stress generation, and spatial and temporal adaptability. Consequently, a comprehensive understanding of the mechanisms underlying the impact of shear stress on physiological and pathological conditions, like thrombosis, remains inadequate. To address these limitations, this study presents a microfluidic-based shear stress generation chip as a proposed solution. The chip achieves a substantial 929-fold variation in shear stress solely by adjusting the degree of constriction in branch channels after PDMS fabrication. Experiments demonstrated that a rapid increase in shear stress up to 1000 dyn/$ cm^{2} $ significantly detached 88.2% cells from the substrate. Long-term exposure (24 h) to shear stress levels below 8.3 dyn/$ cm^{2} $ did not significantly impact cell growth. Furthermore, cells exposed to shear stress levels equal to or greater than 8.3 dyn/$ cm^{2} $ exhibited significant alterations in aspect ratio and orientation, following a normal distribution. This microfluidic chip provides a reliable tool for investigating cellular responses to the wide-ranging shear stress existing in both physiological and pathological flow conditions. Graphical Abstract | ||
| 650 | 4 | |a Biotechnology Microfluidics |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Shear-stress generator |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Cell stress |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Thrombus model |7 (dpeaa)DE-He213 | |
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| 700 | 1 | |a Li, Jingjing |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Chang |e verfasserin |4 aut | |
| 700 | 1 | |a Lu, Huaxiu |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Siyuan |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Tingting |e verfasserin |4 aut | |
| 700 | 1 | |a Guo, Tianruo |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Yonggang |e verfasserin |4 aut | |
| 700 | 1 | |a Jin, Jing |e verfasserin |4 aut | |
| 700 | 1 | |a Yan, Sheng |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Huaying |e verfasserin |4 aut | |
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