Silver nanoparticle-induced in vitro flowering in Dendrocalamus strictus (Roxb.) nees and genetic fidelity assessment of regenerants using molecular markers
Background Dendrocalamus strictus (Roxb.) Nees, generally referred to as ‘Male bamboo,’ is a globally prevalent and highly significant species of bamboo. It is a versatile species and possesses notable industrial significance. However, despite its numerous applications, the production of this plant is insufficient to fulfill the worldwide demand. The challenges that impede the dissemination of D. strictus encompass the unpredictable blooming pattern (30–70 years), low seed production, and limited seed viability. Therefore, tissue culture presents a reliable and effective option for the mass production of standardized planting material. Methodology and results This study investigated the effects of silver nanoparticles (AgNPs) at a concentration of 6.0 mg $ L^{− 1} $ in the Murashige and Skoog (MS) nutrient medium fortified with pre-optimized plant growth regulators (3.0 mg $ L^{− 1} $ 6-benzylaminopurine + 0.5 mg $ L^{− 1} $ α-naphthalene acetic acid) on the induction of flowering in a controlled environment in D. strictus. The use of AgNPs in the media induced a maximum of 14 inflorescences per culture vessel, 9 flowers per inflorescence, and improved the performance of the micropropagated plantlets during acclimatization in the greenhouse and field. The ISSR and SCoT amplified polymorphic DNA analysis of the regenerants resulted in the formation of 49 bands (300 to 2000 bp size) and 36 scorable bands (350 to 2000 bp) respectively. All the PCR amplicons produced by SCoT and ISSR were monomorphic confirming the genetic uniformity of the tissue cultured plants of D. strictus with the mother plant. Conclusions It can be inferred that the incorporation of AgNPs during the shoot proliferation phase has the potential to stimulate in vitro flowering in D. strictus. This finding could provide valuable insights into innovative strategies for enhancing crop productivity and genetic manipulation for accelerated breeding and agricultural advancement..
Key message The utilization of silver nanoparticles in the nutrient medium significantly induced flowering in Dendrocalamus strictus, demonstrating the potential for plant breeding, enhancing crop productivity, and agricultural advancement through tissue culture techniques..
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:51 |
|---|---|
| Enthalten in: |
Molecular biology reports - 51(2024), 1 vom: 10. Apr. |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Rajput, Bharat Singh [VerfasserIn] |
|---|
| Links: |
Volltext [lizenzpflichtig] |
|---|
| BKL: | |
|---|---|
| Themen: |
Flowering |
| Anmerkungen: |
© The Author(s), under exclusive licence to Springer Nature B.V. 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
|---|
| doi: |
10.1007/s11033-024-09472-y |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
SPR055479847 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | SPR055479847 | ||
| 003 | DE-627 | ||
| 005 | 20240411064654.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240411s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s11033-024-09472-y |2 doi | |
| 035 | |a (DE-627)SPR055479847 | ||
| 035 | |a (SPR)s11033-024-09472-y-e | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | 4 | |a 570 |q VZ |
| 084 | |a 42.13 |2 bkl | ||
| 100 | 1 | |a Rajput, Bharat Singh |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Silver nanoparticle-induced in vitro flowering in Dendrocalamus strictus (Roxb.) nees and genetic fidelity assessment of regenerants using molecular markers |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a © The Author(s), under exclusive licence to Springer Nature B.V. 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. | ||
| 520 | |a Background Dendrocalamus strictus (Roxb.) Nees, generally referred to as ‘Male bamboo,’ is a globally prevalent and highly significant species of bamboo. It is a versatile species and possesses notable industrial significance. However, despite its numerous applications, the production of this plant is insufficient to fulfill the worldwide demand. The challenges that impede the dissemination of D. strictus encompass the unpredictable blooming pattern (30–70 years), low seed production, and limited seed viability. Therefore, tissue culture presents a reliable and effective option for the mass production of standardized planting material. Methodology and results This study investigated the effects of silver nanoparticles (AgNPs) at a concentration of 6.0 mg $ L^{− 1} $ in the Murashige and Skoog (MS) nutrient medium fortified with pre-optimized plant growth regulators (3.0 mg $ L^{− 1} $ 6-benzylaminopurine + 0.5 mg $ L^{− 1} $ α-naphthalene acetic acid) on the induction of flowering in a controlled environment in D. strictus. The use of AgNPs in the media induced a maximum of 14 inflorescences per culture vessel, 9 flowers per inflorescence, and improved the performance of the micropropagated plantlets during acclimatization in the greenhouse and field. The ISSR and SCoT amplified polymorphic DNA analysis of the regenerants resulted in the formation of 49 bands (300 to 2000 bp size) and 36 scorable bands (350 to 2000 bp) respectively. All the PCR amplicons produced by SCoT and ISSR were monomorphic confirming the genetic uniformity of the tissue cultured plants of D. strictus with the mother plant. Conclusions It can be inferred that the incorporation of AgNPs during the shoot proliferation phase has the potential to stimulate in vitro flowering in D. strictus. This finding could provide valuable insights into innovative strategies for enhancing crop productivity and genetic manipulation for accelerated breeding and agricultural advancement. | ||
| 520 | |a Key message The utilization of silver nanoparticles in the nutrient medium significantly induced flowering in Dendrocalamus strictus, demonstrating the potential for plant breeding, enhancing crop productivity, and agricultural advancement through tissue culture techniques. | ||
| 650 | 4 | |a Flowering |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a In vitro |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Male bamboo |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Nanoparticles |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Plant growth regulators |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Manokari, M. |e verfasserin |4 aut | |
| 700 | 1 | |a Solanki, Nitin J. |e verfasserin |4 aut | |
| 700 | 1 | |a Sandhya, Dulam |e verfasserin |4 aut | |
| 700 | 1 | |a Faisal, Mohammad |e verfasserin |4 aut | |
| 700 | 1 | |a Alatar, Abdulrahman A. |e verfasserin |4 aut | |
| 700 | 1 | |a Shekhawat, Mahipal S. |e verfasserin |0 (orcid)0000-0002-0990-8766 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Molecular biology reports |d Springer Netherlands, 1973 |g 51(2024), 1 vom: 10. Apr. |w (DE-627)SPR015864855 |w (DE-600)1478217-0 |x 1573-4978 |7 nnns |
| 773 | 1 | 8 | |g volume:51 |g year:2024 |g number:1 |g day:10 |g month:04 |
| 856 | 4 | 0 | |u https://dx.doi.org/10.1007/s11033-024-09472-y |m X:VERLAG |x 0 |z lizenzpflichtig |3 Volltext |
| 912 | |a GBV_SPRINGER | ||
| 912 | |a SSG-OLC-PHA | ||
| 936 | b | k | |a 42.13 |q VZ |
| 951 | |a AR | ||
| 952 | |d 51 |j 2024 |e 1 |b 10 |c 04 | ||