Preparation of fibroblast growth factor 2-incorporated carboxymethyl cellulose nanoparticles for tissue repair and regeneration
Fibroblast growth factor 2 (FGF-2) is a protein that plays an important role in the skin wound healing process. However, the biomedical application of this protein has some limitations due to its instability. To address this problem, this study developed carboxymethyl cellulose (CMC) nanoparticles (NPs) as a nano-carrier for FGF-2 encapsulation and stabilization to improve the bioavailability of this therapeutic protein. Using aluminum chloride ($ AlCl_{3} $) as a cross-linking agent, sphere-shaped CMC NPs were successfully created with a size of 85.60 ± 12.15 nm and no cytotoxicity on NIH/3T3 cell line. In FGF-2 encapsulation, the pre-gelation FGF-2 concentration at 50 μg/mL resulted in the highest FGF-2 loading efficiency at over 90%. FGF-2-incorporated CMC NPs (CMC:FGF-2 NPs) exhibited the same size (88.06 ± 13.51 nm) with CMC NPs, release rates at approximately 30% in aqueous solution after 48 h, preservation of FGF-2 biological activity on NIH/3T3 cell line, and FGF-2 protection from protease hydrolytic action. In burn treatment on mice, CMC:FGF-2 NPs displayed an acceleration of wound closure, growth of granulation tissue, re-epithelialization, and angiogenesis compared to CMC NPs and naked FGF-2. Collectively, this study was a groundwork for the further clinical study of CMC:FGF-2 NPs in burn treatment. Graphical abstract.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:31 |
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| Enthalten in: |
Cellulose - 31(2024), 5 vom: 23. Feb., Seite 2937-2956 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Le, Khanh-Thien [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| BKL: | |
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| Themen: |
Carboxymethyl cellulose |
| Anmerkungen: |
© The Author(s), under exclusive licence to Springer Nature B.V. 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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| doi: |
10.1007/s10570-024-05779-y |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Fibroblast growth factor 2 (FGF-2) is a protein that plays an important role in the skin wound healing process. However, the biomedical application of this protein has some limitations due to its instability. To address this problem, this study developed carboxymethyl cellulose (CMC) nanoparticles (NPs) as a nano-carrier for FGF-2 encapsulation and stabilization to improve the bioavailability of this therapeutic protein. Using aluminum chloride ($ AlCl_{3} $) as a cross-linking agent, sphere-shaped CMC NPs were successfully created with a size of 85.60 ± 12.15 nm and no cytotoxicity on NIH/3T3 cell line. In FGF-2 encapsulation, the pre-gelation FGF-2 concentration at 50 μg/mL resulted in the highest FGF-2 loading efficiency at over 90%. FGF-2-incorporated CMC NPs (CMC:FGF-2 NPs) exhibited the same size (88.06 ± 13.51 nm) with CMC NPs, release rates at approximately 30% in aqueous solution after 48 h, preservation of FGF-2 biological activity on NIH/3T3 cell line, and FGF-2 protection from protease hydrolytic action. In burn treatment on mice, CMC:FGF-2 NPs displayed an acceleration of wound closure, growth of granulation tissue, re-epithelialization, and angiogenesis compared to CMC NPs and naked FGF-2. Collectively, this study was a groundwork for the further clinical study of CMC:FGF-2 NPs in burn treatment. Graphical abstract | ||
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| 700 | 1 | |a Tran-Van, Hieu |4 aut | |
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