Identification and functional analysis of a CbSHR homolog in controlling adventitious root development in Catalpa bungei
Abstract Clonal forestry is an important approach for intensive management as it involves vegetative propagation, which does not entail gene separation and recombination, thereby retaining the excellent traits of the parent trees. Exploring essential genes involved in rooting of cuttings seems urgent in Catalpa bungei as it is the capital method in vegetative propagation. In this study, we identified a homolog of the SHR gene involved in the development of adventitious roots (ARs) in C. bungei through multiple alignment, homologous cloning, qPCR detection, and transgenic techniques. The CbSHR gene encodes a protein of 445 amino acids, and the expression of the CbSHR gene reaches its peak at the callus differentiation stage (S4, about 30 days after cutting) during AR development. By overexpressing the CbSHR gene in tobacco, the number of roots and their length were increased compared with the wild-type line, thus identifying a pivotal homolog, the CbSHR gene in C. bungei, which promotes the initiation and elongation of ARs. This result provided a candidate gene for genetic improvement of cutting, and will also contribute to understanding the molecular mechanisms of AR development in C. bungei..
Key Message In this study, we identified a homolog of CbSHR gene in C. bungei through multiple alignment, homologous cloning, qPCR detection, and transgenic techniques. The CbSHR gene encodes a protein of 445 amino acids, the expression of the CbSHR gene reaches its peak at the callus differentiation stage during branches cutting. Heterologous overexpression of CbSHR gene in tobacco displays a booming ARs, including RN (number of roots) and RL (root length) when compared with the wild-type line. Thus, we identified a pivotal homolog of CbSHR gene in C. bungei, which promoted the initiation and elongation of ARs. This result provided a candidate gene for genetic improvement of cutting, and will also contribute to understanding the molecular mechanisms of AR development in C. bungei..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:157 |
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| Enthalten in: |
Plant cell, tissue and organ culture - 157(2024), 1 vom: 27. März |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Hao, Ziyuan [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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© The Author(s), under exclusive licence to Springer Nature B.V. 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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| doi: |
10.1007/s11240-024-02730-8 |
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| 520 | |a Abstract Clonal forestry is an important approach for intensive management as it involves vegetative propagation, which does not entail gene separation and recombination, thereby retaining the excellent traits of the parent trees. Exploring essential genes involved in rooting of cuttings seems urgent in Catalpa bungei as it is the capital method in vegetative propagation. In this study, we identified a homolog of the SHR gene involved in the development of adventitious roots (ARs) in C. bungei through multiple alignment, homologous cloning, qPCR detection, and transgenic techniques. The CbSHR gene encodes a protein of 445 amino acids, and the expression of the CbSHR gene reaches its peak at the callus differentiation stage (S4, about 30 days after cutting) during AR development. By overexpressing the CbSHR gene in tobacco, the number of roots and their length were increased compared with the wild-type line, thus identifying a pivotal homolog, the CbSHR gene in C. bungei, which promotes the initiation and elongation of ARs. This result provided a candidate gene for genetic improvement of cutting, and will also contribute to understanding the molecular mechanisms of AR development in C. bungei. | ||
| 520 | |a Key Message In this study, we identified a homolog of CbSHR gene in C. bungei through multiple alignment, homologous cloning, qPCR detection, and transgenic techniques. The CbSHR gene encodes a protein of 445 amino acids, the expression of the CbSHR gene reaches its peak at the callus differentiation stage during branches cutting. Heterologous overexpression of CbSHR gene in tobacco displays a booming ARs, including RN (number of roots) and RL (root length) when compared with the wild-type line. Thus, we identified a pivotal homolog of CbSHR gene in C. bungei, which promoted the initiation and elongation of ARs. This result provided a candidate gene for genetic improvement of cutting, and will also contribute to understanding the molecular mechanisms of AR development in C. bungei. | ||
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