NT-proBNP detection with a one-step magnetic lateral flow channel assay
Point-of-care sensors targeting blood marker analysis must be designed to function with very small volumes since acquiring a blood sample through a simple, mostly pain-free finger prick dramatically limits the sample size and comforts the patient. Therefore, we explored the potential of converting a conventional lateral flow assay (LFA) for a significant biomarker into a self-contained and compact polymer channel-based LFA to minimize the sample volume while maintaining the analytical merits. Our primary objective was to eliminate the use of sample-absorbing fleece and membrane materials commonly present in LFAs. Simultaneously, we concentrated on developing a ready-to-deploy one-step LFA format, characterized by dried reagents, facilitating automation and precise sample transport through a pump control system. We targeted the detection of the heart failure biomarker NT-proBNP in only 15 µL human whole blood and therefore implemented strategies that ensure highly sensitive detection. The biosensor combines streptavidin-functionalized magnetic beads (MNPs) as a 3D detection zone and fluorescence nanoparticles as signal labels in a sandwich-based immunoassay. Compared to the currently commercialized LFA, our biosensor demonstrates comparable analytical performance with only a tenth of the sample volume. With a detection limit of 43.1 pg∙$ mL^{−1} $ and a mean error of 18% (n ≥ 3), the biosensor offers high sensitivity and accuracy. The integration of all-dried long-term stable reagents further enhances the convenience and stability of the biosensor. This lateral flow channel platform represents a promising advancement in point-of-care diagnostics for heart failure biomarkers, offering a user-friendly and sensitive platform for rapid and reliable testing with low finger-prick blood sample volumes. Graphical abstract.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:416 |
|---|---|
| Enthalten in: |
Analytical and bioanalytical chemistry - 416(2024), 10 vom: 08. März, Seite 2411-2422 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Strohmaier-Nguyen, Dan [VerfasserIn] |
|---|
| Links: |
Volltext [kostenfrei] |
|---|
| BKL: | |
|---|---|
| Themen: |
Cardiac |
| Anmerkungen: |
© The Author(s) 2024 |
|---|
| doi: |
10.1007/s00216-024-05223-x |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
SPR055315283 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | SPR055315283 | ||
| 003 | DE-627 | ||
| 005 | 20240328072112.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240328s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s00216-024-05223-x |2 doi | |
| 035 | |a (DE-627)SPR055315283 | ||
| 035 | |a (SPR)s00216-024-05223-x-e | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | 4 | |a 540 |a 570 |q VZ |
| 082 | 0 | 4 | |a 540 |q VZ |
| 084 | |a 35.23 |2 bkl | ||
| 084 | |a 35.71 |2 bkl | ||
| 084 | |a 42.03 |2 bkl | ||
| 100 | 1 | |a Strohmaier-Nguyen, Dan |e verfasserin |0 (orcid)0009-0003-9458-4990 |4 aut | |
| 245 | 1 | 0 | |a NT-proBNP detection with a one-step magnetic lateral flow channel assay |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a © The Author(s) 2024 | ||
| 520 | |a Point-of-care sensors targeting blood marker analysis must be designed to function with very small volumes since acquiring a blood sample through a simple, mostly pain-free finger prick dramatically limits the sample size and comforts the patient. Therefore, we explored the potential of converting a conventional lateral flow assay (LFA) for a significant biomarker into a self-contained and compact polymer channel-based LFA to minimize the sample volume while maintaining the analytical merits. Our primary objective was to eliminate the use of sample-absorbing fleece and membrane materials commonly present in LFAs. Simultaneously, we concentrated on developing a ready-to-deploy one-step LFA format, characterized by dried reagents, facilitating automation and precise sample transport through a pump control system. We targeted the detection of the heart failure biomarker NT-proBNP in only 15 µL human whole blood and therefore implemented strategies that ensure highly sensitive detection. The biosensor combines streptavidin-functionalized magnetic beads (MNPs) as a 3D detection zone and fluorescence nanoparticles as signal labels in a sandwich-based immunoassay. Compared to the currently commercialized LFA, our biosensor demonstrates comparable analytical performance with only a tenth of the sample volume. With a detection limit of 43.1 pg∙$ mL^{−1} $ and a mean error of 18% (n ≥ 3), the biosensor offers high sensitivity and accuracy. The integration of all-dried long-term stable reagents further enhances the convenience and stability of the biosensor. This lateral flow channel platform represents a promising advancement in point-of-care diagnostics for heart failure biomarkers, offering a user-friendly and sensitive platform for rapid and reliable testing with low finger-prick blood sample volumes. Graphical abstract | ||
| 650 | 4 | |a Magnetic bead |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Lateral flow assay |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Immunoassay |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Cardiac |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a One-step |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Point-of-care |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Horn, Carina |4 aut | |
| 700 | 1 | |a Baeumner, Antje J. |0 (orcid)0000-0001-7148-3423 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Analytical and bioanalytical chemistry |d Springer Berlin Heidelberg, 2002 |g 416(2024), 10 vom: 08. März, Seite 2411-2422 |w (DE-627)SPR002080982 |w (DE-600)1459122-4 |x 1618-2650 |7 nnns |
| 773 | 1 | 8 | |g volume:416 |g year:2024 |g number:10 |g day:08 |g month:03 |g pages:2411-2422 |
| 856 | 4 | 0 | |u https://dx.doi.org/10.1007/s00216-024-05223-x |z kostenfrei |3 Volltext |
| 912 | |a GBV_SPRINGER | ||
| 912 | |a SSG-OLC-PHA | ||
| 936 | b | k | |a 35.23 |q VZ |
| 936 | b | k | |a 35.71 |q VZ |
| 936 | b | k | |a 42.03 |q VZ |
| 951 | |a AR | ||
| 952 | |d 416 |j 2024 |e 10 |b 08 |c 03 |h 2411-2422 | ||