Evaluation of next-generation sequencing versus next-generation flow cytometry for minimal-residual-disease detection in Chinese patients with multiple myeloma
Purpose To evaluate the efficacy of next-generation sequencing (NGS) in minimal-residual-disease (MRD) monitoring in Chinese patients with multiple myeloma (MM). Methods This study analyzed 60 Chinese MM patients. During MRD monitoring in these patients’ post-therapy, clonal immunoglobulin heavy chain (IGH) rearrangements were detected via NGS using LymphoTrack assays. MRD monitoring was performed using NGS or next-generation flow cytometry (NGF), and the results were compared. Additionally, the sensitivity and reproducibility of the NGS method were assessed. Results The MRD detection range of the NGS method was $ 10^{–6} $–$ 10^{–1} $, which suggested good linearity, with a Pearson correlation coefficient of 0.985 and a limit of detection of $ 10^{–6} $. Intra- and inter-assay reproducibility analyses showed that NGS exhibited 100% reproducibility with low variability in clonal cells. At diagnosis, unique clones were found in 42 patients (70.0%) with clonal IGH rearrangements, which were used as clonality markers for MRD monitoring post-therapy. Comparison of NGS and NGF for MRD monitoring showed 79.1% concordance. No samples that tested MRD-positive via NGF were found negative via NGS, indicating the higher sensitivity of NGS. MRD could be detected using NGS in 6 of 7 samples before autologous hematopoietic stem-cell transplantation, and 5 of them tested negative post-transplantation. In contrast, the NGF method could detect MRD in only 1 sample pre-transplantation. Conclusion Compared with NGF, NGS exhibits higher sensitivity and reproducibility in MRD detection and can be an effective strategy for MRD monitoring in Chinese MM patients..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:15 |
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| Enthalten in: |
Discover oncology - 15(2024), 1 vom: 19. März |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhou, Mo [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| Themen: |
IGH gene rearrangement |
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| Anmerkungen: |
© The Author(s) 2024 |
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| doi: |
10.1007/s12672-024-00938-w |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
SPR055214789 |
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| 520 | |a Purpose To evaluate the efficacy of next-generation sequencing (NGS) in minimal-residual-disease (MRD) monitoring in Chinese patients with multiple myeloma (MM). Methods This study analyzed 60 Chinese MM patients. During MRD monitoring in these patients’ post-therapy, clonal immunoglobulin heavy chain (IGH) rearrangements were detected via NGS using LymphoTrack assays. MRD monitoring was performed using NGS or next-generation flow cytometry (NGF), and the results were compared. Additionally, the sensitivity and reproducibility of the NGS method were assessed. Results The MRD detection range of the NGS method was $ 10^{–6} $–$ 10^{–1} $, which suggested good linearity, with a Pearson correlation coefficient of 0.985 and a limit of detection of $ 10^{–6} $. Intra- and inter-assay reproducibility analyses showed that NGS exhibited 100% reproducibility with low variability in clonal cells. At diagnosis, unique clones were found in 42 patients (70.0%) with clonal IGH rearrangements, which were used as clonality markers for MRD monitoring post-therapy. Comparison of NGS and NGF for MRD monitoring showed 79.1% concordance. No samples that tested MRD-positive via NGF were found negative via NGS, indicating the higher sensitivity of NGS. MRD could be detected using NGS in 6 of 7 samples before autologous hematopoietic stem-cell transplantation, and 5 of them tested negative post-transplantation. In contrast, the NGF method could detect MRD in only 1 sample pre-transplantation. Conclusion Compared with NGF, NGS exhibits higher sensitivity and reproducibility in MRD detection and can be an effective strategy for MRD monitoring in Chinese MM patients. | ||
| 650 | 4 | |a Multiple myeloma |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Minimal residual disease |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Next-generation sequencing |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a IGH gene rearrangement |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Next-generation flow cytometry |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Chen, Yan |4 aut | |
| 700 | 1 | |a Gong, Yanlei |4 aut | |
| 700 | 1 | |a Zhu, Mingqing |4 aut | |
| 700 | 1 | |a Cen, Jiannong |4 aut | |
| 700 | 1 | |a Pan, Jinlan |4 aut | |
| 700 | 1 | |a Yan, Lingzhi |4 aut | |
| 700 | 1 | |a Shang, Jingjing |4 aut | |
| 700 | 1 | |a Jin, Song |4 aut | |
| 700 | 1 | |a Shi, Xiaolan |4 aut | |
| 700 | 1 | |a Yao, Weiqin |4 aut | |
| 700 | 1 | |a Yan, Shuang |4 aut | |
| 700 | 1 | |a Wu, Depei |4 aut | |
| 700 | 1 | |a Chen, Suning |4 aut | |
| 700 | 1 | |a Fu, Chengcheng |4 aut | |
| 700 | 1 | |a Yao, Li |0 (orcid)0000-0001-5842-3768 |4 aut | |
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