Assembly and characterization of the complete mitochondrial genome of Descurainia sophia: a medical plant producing glucosinolate
Abstract As a commonly used traditional Chinese medicine, Descurainia sophia has various reported pharmacological activities such as prevention of chronic diseases, cardiovascular protection, neuroprotection, anticancer, antioxidant, and has high nutritional values, but its mitochondrial genome (mitogenome) has not been reported. In this study, the D. sophia mitogenome was sequenced, assembled and annotated using next generation sequencing technologies on the Illumina HiSeq and PacBio Sequel platform. The results showed that the D. sophia mitogenome was a circular DNA molecule with the length of 265,457 bp and the GC content of 44.78%, encoding unique 56 genes, including 35 protein coding genes, 18 tRNA genes and 3 rRNA genes. We analyzed codon preference, repeated sequences, DNA sequence transformation, phylogenetic relationship, RNA editing and synteny in the D. sophia mitogenome. Based on the DNA sequences of mitogenomes, the phylogenetic and synteny analysis indicated that D. sophia was more closely related to Boechera stricta, and the mitogenome of D. sophia underwent frequent genome recombination. In this study, we obtained the complete mitogenome of D. sophia, which can provide valuable data support for future studies on genetic characteristics, phylogenetic relationships, species identification and development of new molecular markers in D. sophia..
Article Highlights The results of this study provide valuable information about the mitogenome of D. sophia, and will contribute to the research of evolution and mitogenome variation in the genus Descurainia.The D. sophia mitogenome was an unbranched circular DNA molecule, and the synteny analysis revealed that genomic recombination occurred frequently in the mitogenome of D. sophia, which was evolutionarily closest related to Boechera stricta.A total of 57 SSRs and 406 potential C → U RNA editing sites were identified in mitogenome, which can be used to develop new molecular markers..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:6 |
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| Enthalten in: |
Discover applied sciences - 6(2024), 3 vom: 13. März |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhao, Le [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| Themen: |
Mitochondrial genome |
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| Anmerkungen: |
© The Author(s) 2024 |
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| doi: |
10.1007/s42452-024-05775-8 |
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| funding: |
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| PPN (Katalog-ID): |
SPR055138462 |
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| 520 | |a Abstract As a commonly used traditional Chinese medicine, Descurainia sophia has various reported pharmacological activities such as prevention of chronic diseases, cardiovascular protection, neuroprotection, anticancer, antioxidant, and has high nutritional values, but its mitochondrial genome (mitogenome) has not been reported. In this study, the D. sophia mitogenome was sequenced, assembled and annotated using next generation sequencing technologies on the Illumina HiSeq and PacBio Sequel platform. The results showed that the D. sophia mitogenome was a circular DNA molecule with the length of 265,457 bp and the GC content of 44.78%, encoding unique 56 genes, including 35 protein coding genes, 18 tRNA genes and 3 rRNA genes. We analyzed codon preference, repeated sequences, DNA sequence transformation, phylogenetic relationship, RNA editing and synteny in the D. sophia mitogenome. Based on the DNA sequences of mitogenomes, the phylogenetic and synteny analysis indicated that D. sophia was more closely related to Boechera stricta, and the mitogenome of D. sophia underwent frequent genome recombination. In this study, we obtained the complete mitogenome of D. sophia, which can provide valuable data support for future studies on genetic characteristics, phylogenetic relationships, species identification and development of new molecular markers in D. sophia. | ||
| 520 | |a Article Highlights The results of this study provide valuable information about the mitogenome of D. sophia, and will contribute to the research of evolution and mitogenome variation in the genus Descurainia.The D. sophia mitogenome was an unbranched circular DNA molecule, and the synteny analysis revealed that genomic recombination occurred frequently in the mitogenome of D. sophia, which was evolutionarily closest related to Boechera stricta.A total of 57 SSRs and 406 potential C → U RNA editing sites were identified in mitogenome, which can be used to develop new molecular markers. | ||
| 650 | 4 | |a Mitochondrial genome |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Repeated sequences |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Phylogenetic analysis |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a RNA editing |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Yan, Hang |4 aut | |
| 700 | 1 | |a Zhu, Yun-hao |4 aut | |
| 700 | 1 | |a Jia, Hao-yu |4 aut | |
| 700 | 1 | |a Han, Yong-guang |4 aut | |
| 700 | 1 | |a Zheng, Xiao-ke |4 aut | |
| 700 | 1 | |a Wang, Min |4 aut | |
| 700 | 1 | |a Feng, Wei-sheng |4 aut | |
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