Differences in PPD- and mitogen-induced T-cell activation marker expression characterize immunopathology in acute tuberculosis patients
Abstract Impaired T-cell responses to mitogens and high T-cell activation marker (TAM) expression on Mycobacterium tuberculosis–specific T-cells characterize immunopathology in patients with tuberculosis (TB). In a study of patients with TB (n = 60) and asymptomatic contacts (controls, n = 37), we found that TB patients had higher $ CD38^{+} $ T-cell proportions specific for M. tuberculosis protein ($ PPD_{Mtb} $), yet total proportions of $ PPD_{Mtb} $-specific T-cells were comparable. Notably, both activated ($ CD38^{+} $) and total IFN-$ γ^{+} $ T-cells from TB patients had lower mitogen (phytohemagglutinin, PHA)-induced responses. This impaired mitogen response improved the classification efficacy of the TAM-TB assay, especially employing the PPD/PHA-induced T-cell ratio..
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:43 |
|---|---|
| Enthalten in: |
European journal of clinical microbiology & infectious diseases - 43(2024), 3 vom: 03. Jan., Seite 611-616 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Acheampong, Isaac [VerfasserIn] |
|---|
| Links: |
Volltext [kostenfrei] |
|---|
| Themen: |
Anergy |
|---|
| Anmerkungen: |
© The Author(s) 2024 |
|---|
| doi: |
10.1007/s10096-023-04741-3 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
SPR055040357 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | SPR055040357 | ||
| 003 | DE-627 | ||
| 005 | 20240307064641.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240307s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s10096-023-04741-3 |2 doi | |
| 035 | |a (DE-627)SPR055040357 | ||
| 035 | |a (SPR)s10096-023-04741-3-e | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Acheampong, Isaac |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Differences in PPD- and mitogen-induced T-cell activation marker expression characterize immunopathology in acute tuberculosis patients |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a © The Author(s) 2024 | ||
| 520 | |a Abstract Impaired T-cell responses to mitogens and high T-cell activation marker (TAM) expression on Mycobacterium tuberculosis–specific T-cells characterize immunopathology in patients with tuberculosis (TB). In a study of patients with TB (n = 60) and asymptomatic contacts (controls, n = 37), we found that TB patients had higher $ CD38^{+} $ T-cell proportions specific for M. tuberculosis protein ($ PPD_{Mtb} $), yet total proportions of $ PPD_{Mtb} $-specific T-cells were comparable. Notably, both activated ($ CD38^{+} $) and total IFN-$ γ^{+} $ T-cells from TB patients had lower mitogen (phytohemagglutinin, PHA)-induced responses. This impaired mitogen response improved the classification efficacy of the TAM-TB assay, especially employing the PPD/PHA-induced T-cell ratio. | ||
| 650 | 4 | |a Tuberculosis |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Immunopathology |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Mitogen |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a T-cell activation marker |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Anergy |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Minadzi, Difery |4 aut | |
| 700 | 1 | |a Laing, Edwin F. |4 aut | |
| 700 | 1 | |a Frimpong, Michael |4 aut | |
| 700 | 1 | |a Vivekanandan, Monika M. |4 aut | |
| 700 | 1 | |a Yeboah, Augustine |4 aut | |
| 700 | 1 | |a Adankwah, Ernest |4 aut | |
| 700 | 1 | |a Aniagyei, Wilfred |4 aut | |
| 700 | 1 | |a Arthur, Joseph F. |4 aut | |
| 700 | 1 | |a Lamptey, Millicent |4 aut | |
| 700 | 1 | |a Abass, Mohammed K. |4 aut | |
| 700 | 1 | |a Kumbel, Francis |4 aut | |
| 700 | 1 | |a Osei-Yeboah, Francis |4 aut | |
| 700 | 1 | |a Gawusu, Amidu |4 aut | |
| 700 | 1 | |a Debrah, Linda Batsa |4 aut | |
| 700 | 1 | |a Owusu, Dorcas O. |4 aut | |
| 700 | 1 | |a Debrah, Alexander |4 aut | |
| 700 | 1 | |a Mayatepek, Ertan |4 aut | |
| 700 | 1 | |a Seyfarth, Julia |4 aut | |
| 700 | 1 | |a Phillips, Richard O. |4 aut | |
| 700 | 1 | |a Jacobsen, Marc |0 (orcid)0000-0002-4703-5652 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t European journal of clinical microbiology & infectious diseases |d Berlin : Springer, 1982 |g 43(2024), 3 vom: 03. Jan., Seite 611-616 |w (DE-627)SPR008661995 |w (DE-600)1459049-9 |x 1435-4373 |7 nnns |
| 773 | 1 | 8 | |g volume:43 |g year:2024 |g number:3 |g day:03 |g month:01 |g pages:611-616 |
| 856 | 4 | 0 | |u https://dx.doi.org/10.1007/s10096-023-04741-3 |z kostenfrei |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_SPRINGER | ||
| 951 | |a AR | ||
| 952 | |d 43 |j 2024 |e 3 |b 03 |c 01 |h 611-616 | ||