Paper-based uric acid assay in whole blood samples by $ Zn^{2+} $ protein precipitation and enzyme-free colorimetric detection
Uric acid (UA) is an important biomarker, as a high concentration in blood can lead to gout and further renal syndrome. Although several point-of-care testing (POCT) devices have been reported to detect UA, there are some limitations such as the requirement for uricase and the complicated pretreatment of serum/plasma samples, which restricts their use at home or in undeveloped areas. In this work, we developed an approach by applying $ Zn^{2+} $ to precipitate proteins and cells in whole blood to avoid interference with the chromogenic reaction. We used carboxymethylcellulose (CMC) to immobilize tetramethylbenzidine (TMB) on a nitrocellulose membrane for colorimetric detection. Using the oxidization properties of $ H_{2} %$ O_{2} $, which turns TMB into oxidized tetramethylbenzidine (TMBox) in the presence of catalyst gold nanoparticles (AuNPs), we successfully constructed an enzyme-free paper-based POCT device using the reduction reaction of UA and TMBox for simple, speedy, and cheap colorimetric detection of UA, achieving a detection time of 8 min, a linear range of 0–150 μg/mL, and an LOD of 25.79 μg/mL. The UA concentration in whole blood samples was further measured and correlated well with the clinical value (R2 = 0.8212). Thus, the proposed assay has the potential for POCT diagnosis, monitoring, and prognosis of diseases related to UA. Graphical abstract By applying $ Zn^{2+} $ reagent to precipitate proteins and cells in whole blood and the reduction reaction of UA, an enzyme-free paper-based POCT assay was established..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:416 |
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| Enthalten in: |
Analytical and bioanalytical chemistry - 416(2024), 7 vom: 30. Jan., Seite 1589-1597 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Jin, Xue [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| Themen: |
Colorimetric detection |
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| Anmerkungen: |
© The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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| doi: |
10.1007/s00216-024-05160-9 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Uric acid (UA) is an important biomarker, as a high concentration in blood can lead to gout and further renal syndrome. Although several point-of-care testing (POCT) devices have been reported to detect UA, there are some limitations such as the requirement for uricase and the complicated pretreatment of serum/plasma samples, which restricts their use at home or in undeveloped areas. In this work, we developed an approach by applying $ Zn^{2+} $ to precipitate proteins and cells in whole blood to avoid interference with the chromogenic reaction. We used carboxymethylcellulose (CMC) to immobilize tetramethylbenzidine (TMB) on a nitrocellulose membrane for colorimetric detection. Using the oxidization properties of $ H_{2} %$ O_{2} $, which turns TMB into oxidized tetramethylbenzidine (TMBox) in the presence of catalyst gold nanoparticles (AuNPs), we successfully constructed an enzyme-free paper-based POCT device using the reduction reaction of UA and TMBox for simple, speedy, and cheap colorimetric detection of UA, achieving a detection time of 8 min, a linear range of 0–150 μg/mL, and an LOD of 25.79 μg/mL. The UA concentration in whole blood samples was further measured and correlated well with the clinical value (R2 = 0.8212). Thus, the proposed assay has the potential for POCT diagnosis, monitoring, and prognosis of diseases related to UA. Graphical abstract By applying $ Zn^{2+} $ reagent to precipitate proteins and cells in whole blood and the reduction reaction of UA, an enzyme-free paper-based POCT assay was established. | ||
| 650 | 4 | |a Uric acid |7 (dpeaa)DE-He213 | |
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| 700 | 1 | |a Gao, Mingxia |4 aut | |
| 700 | 1 | |a Zhang, Xiangmin |4 aut | |
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