Highly sensitive SERS platform for pathogen analysis by cyclic DNA nanostructureAuNP tags and cascade primer exchange reaction
Abstract The capacity to identify small amounts of pathogens in real samples is extremely useful. Herein, we proposed a sensitive platform for detecting pathogens using cyclic DNA nanostructureAuNP tags (CDNA) and a cascade primer exchange reaction (cPER). This platform employs wheat germ agglutinin-modified $ Fe_{3} %$ O_{4} $@Au magnetic nanoparticles (WMRs) to bind the E. coli O157:H7, and then triggers the cPER to generate branched DNA products for CDNA tag hybridization with high stability and amplified SERS signals. It can identify target pathogens as low as 1.91 CFU/mL and discriminate E. coli O157:H7 in complex samples such as water, milk, and serum, demonstrating comparable or greater sensitivity and accuracy than traditional qPCR. Moreover, the developed platform can detect low levels of E. coli O157:H7 in mouse serum, allowing the discrimination of mice with early-stage infection. Thus, this platform holds promise for food analysis and early infection diagnosis..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:22 |
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| Enthalten in: |
Journal of nanobiotechnology - 22(2024), 1 vom: 26. Feb. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Xiao, Yunju [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| Themen: |
Bacterial detection |
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| Anmerkungen: |
© The Author(s) 2024 |
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| doi: |
10.1186/s12951-024-02339-1 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
SPR054911605 |
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| 520 | |a Abstract The capacity to identify small amounts of pathogens in real samples is extremely useful. Herein, we proposed a sensitive platform for detecting pathogens using cyclic DNA nanostructureAuNP tags (CDNA) and a cascade primer exchange reaction (cPER). This platform employs wheat germ agglutinin-modified $ Fe_{3} %$ O_{4} $@Au magnetic nanoparticles (WMRs) to bind the E. coli O157:H7, and then triggers the cPER to generate branched DNA products for CDNA tag hybridization with high stability and amplified SERS signals. It can identify target pathogens as low as 1.91 CFU/mL and discriminate E. coli O157:H7 in complex samples such as water, milk, and serum, demonstrating comparable or greater sensitivity and accuracy than traditional qPCR. Moreover, the developed platform can detect low levels of E. coli O157:H7 in mouse serum, allowing the discrimination of mice with early-stage infection. Thus, this platform holds promise for food analysis and early infection diagnosis. | ||
| 650 | 4 | |a SERS tags |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Primer exchange reaction |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Bacterial detection |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Early infection diagnosis |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Luo, Shihua |4 aut | |
| 700 | 1 | |a Qiu, Jiuxiang |4 aut | |
| 700 | 1 | |a Zhang, Ye |4 aut | |
| 700 | 1 | |a Liu, Weijiang |4 aut | |
| 700 | 1 | |a Zhao, Yunhu |4 aut | |
| 700 | 1 | |a Zhu, YiTong |4 aut | |
| 700 | 1 | |a Deng, Yangxi |4 aut | |
| 700 | 1 | |a Lu, Mengdi |4 aut | |
| 700 | 1 | |a Liu, Suling |4 aut | |
| 700 | 1 | |a Lin, Yong |4 aut | |
| 700 | 1 | |a Huang, Aiwei |4 aut | |
| 700 | 1 | |a Wang, Wen |4 aut | |
| 700 | 1 | |a Hu, Xuejiao |4 aut | |
| 700 | 1 | |a Gu, Bing |4 aut | |
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