Sphingomicrobium clamense sp. nov., Isolated from Sediment of Clam Island Beach in China
Abstract A Gram-stain-negative, non-flagellated, aerobic, ovoid or rod-shaped bacterium with motility, designated $ B8^{T} $, was isolated from the sediment of Clam Island beach, Liaoning province, China. The optimum growth of strain $ B8^{T} $ occurred at 35 oC, pH 7.0, and in the presence of 4.0–5.0% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain $ B8^{T} $ formed a distinct lineage within the genus Sphingomicrobium and was closely related to Sphingomicrobium nitratireducens O-$ 35^{T} $ (98.3% sequence similarity), Sphingomicrobium aestuariivivum KCTC $ 42286^{T} $ (96.9%), and Sphingomicrobium astaxanthinifaciens JCM $ 18551^{T} $ (96.5%). The digital DNA–DNA hybridization and average nucleotide identity values between strain $ B8^{T} $ and closely related strains were lower than 21.0% and 78.0%, much lower than the cutoff values of 70.0% and 95.0%, respectively, for bacterial species delineation. The dominant respiratory quinone of strain $ B8^{T} $ was ubiquinone-10. The major fatty acids were Sum In Feature 8 ($ C_{18:1} $ω7c and/or $ C_{18:1} $ω6c), Sum In Feature 3 ($ C_{16 : 1} $ω7c and/or $ C_{16 : 1} $ω6c), $ C_{17:1} $ω6c, $ C_{18:1} $ 2-OH, and $ C_{16:0} $. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid, glycolipids, and four unknown polar lipids. The DNA G + C content of strain $ B8^{T} $ was 63.9%. Based on the phenotypic, phylogenetic, and chemotaxonomic analyses, strain $ B8^{T} $ is considered a new species of Sphingomicrobium, for which the name Sphingomicrobium clamense sp. nov. is proposed. The type strain is $ B8^{T} $ (= CGMCC 1.$ 19486^{T} $ = KCTC $ 92052^{T} $)..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:81 |
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| Enthalten in: |
Current microbiology - 81(2024), 4 vom: 23. Feb. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Li, Xing [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| Anmerkungen: |
© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2024 |
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| doi: |
10.1007/s00284-024-03639-3 |
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| 520 | |a Abstract A Gram-stain-negative, non-flagellated, aerobic, ovoid or rod-shaped bacterium with motility, designated $ B8^{T} $, was isolated from the sediment of Clam Island beach, Liaoning province, China. The optimum growth of strain $ B8^{T} $ occurred at 35 oC, pH 7.0, and in the presence of 4.0–5.0% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain $ B8^{T} $ formed a distinct lineage within the genus Sphingomicrobium and was closely related to Sphingomicrobium nitratireducens O-$ 35^{T} $ (98.3% sequence similarity), Sphingomicrobium aestuariivivum KCTC $ 42286^{T} $ (96.9%), and Sphingomicrobium astaxanthinifaciens JCM $ 18551^{T} $ (96.5%). The digital DNA–DNA hybridization and average nucleotide identity values between strain $ B8^{T} $ and closely related strains were lower than 21.0% and 78.0%, much lower than the cutoff values of 70.0% and 95.0%, respectively, for bacterial species delineation. The dominant respiratory quinone of strain $ B8^{T} $ was ubiquinone-10. The major fatty acids were Sum In Feature 8 ($ C_{18:1} $ω7c and/or $ C_{18:1} $ω6c), Sum In Feature 3 ($ C_{16 : 1} $ω7c and/or $ C_{16 : 1} $ω6c), $ C_{17:1} $ω6c, $ C_{18:1} $ 2-OH, and $ C_{16:0} $. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid, glycolipids, and four unknown polar lipids. The DNA G + C content of strain $ B8^{T} $ was 63.9%. Based on the phenotypic, phylogenetic, and chemotaxonomic analyses, strain $ B8^{T} $ is considered a new species of Sphingomicrobium, for which the name Sphingomicrobium clamense sp. nov. is proposed. The type strain is $ B8^{T} $ (= CGMCC 1.$ 19486^{T} $ = KCTC $ 92052^{T} $). | ||
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