Development of an electrochemical sensitive aptasensor based on a zeolite imidazolate framework-8 and gold nanoparticles for the determination of Staphylococcus aureus bacteria
Abstract Staphylococcus aureus (S. aureus) is one of the most important pathogens that cause illness and food poisoning. In this research, using a glassy carbon electrode (GCE) modified with zeolite imidazolate framework-8 (ZIF 8) and gold nanoparticles (AuNPs), a sensitive electrochemical aptasensor has been made for the detection of the S. aureus bacteria. The morphology of the prepared AuNPs-ZIF 8 nanocomposite has been carefully characterized by means of transmission electron microscopy (TEM), field emission scanning electron microscopy (FESEM), and energy-dispersive X-ray spectroscopy (EDS). In the manufacturing process, the S. aureus aptamer is immobilized on the AuNPs-ZIF 8 surface. Electrochemical impedance spectroscopy (EIS) method has been used for quantitative determination of S. aureus bacteria. The changes in the charge transfer resistance (Rct) of the aptamer due to the change in the concentration of bacteria are considered as the analytical signals. The proposed aptasensor has linear response in the concentration range of 1.5 × $ 10^{1} $ to 1.5 × $ 10^{7} $ CFU $ mL^{−1} $ of S. aureus bacteria. The detection limit of the method is 3.4 CFU $ mL^{−1} $. Using the developed aptasensor, it is possible to determine S. aureus bacteria in water and milk samples..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:416 |
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| Enthalten in: |
Analytical and bioanalytical chemistry - 416(2024), 5 vom: 05. Jan., Seite 1229-1238 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Morsalpour, Hafezeh [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| Themen: |
Aptamer |
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| Anmerkungen: |
© The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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| doi: |
10.1007/s00216-023-05115-6 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Abstract Staphylococcus aureus (S. aureus) is one of the most important pathogens that cause illness and food poisoning. In this research, using a glassy carbon electrode (GCE) modified with zeolite imidazolate framework-8 (ZIF 8) and gold nanoparticles (AuNPs), a sensitive electrochemical aptasensor has been made for the detection of the S. aureus bacteria. The morphology of the prepared AuNPs-ZIF 8 nanocomposite has been carefully characterized by means of transmission electron microscopy (TEM), field emission scanning electron microscopy (FESEM), and energy-dispersive X-ray spectroscopy (EDS). In the manufacturing process, the S. aureus aptamer is immobilized on the AuNPs-ZIF 8 surface. Electrochemical impedance spectroscopy (EIS) method has been used for quantitative determination of S. aureus bacteria. The changes in the charge transfer resistance (Rct) of the aptamer due to the change in the concentration of bacteria are considered as the analytical signals. The proposed aptasensor has linear response in the concentration range of 1.5 × $ 10^{1} $ to 1.5 × $ 10^{7} $ CFU $ mL^{−1} $ of S. aureus bacteria. The detection limit of the method is 3.4 CFU $ mL^{−1} $. Using the developed aptasensor, it is possible to determine S. aureus bacteria in water and milk samples. | ||
| 650 | 4 | |a Aptamer |7 (dpeaa)DE-He213 | |
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| 700 | 1 | |a Shekari, Zahra |4 aut | |
| 700 | 1 | |a Mirbagheri, Maryam |4 aut | |
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