MenSCs Transplantation Improve the Viability of Injured Endometrial Cells Through Activating PI3K/Akt Pathway
Abstract Endometrial injury is one of the leading causes of female infertility and is caused by intrauterine surgery, endometrial infection, repeated abortion, or genital tuberculosis. Currently, there is little effective treatment to restore the fertility of patients with severe intrauterine adhesions and thin endometrium. Recent studies have confirmed the promising therapeutic effects of mesenchymal stem cell transplantation on various diseases with definite tissue injury. The aim of this study is to investigate the improvements of menstrual blood-derived endometrial stem cells (MenSCs) transplantation on functional restoration in the endometrium of mouse model. Therefore, ethanol-induced endometrial injury mouse models were randomly divided into two groups: the PBS-treated group, and the MenSCs-treated group. As expected, the endometrial thickness and gland number in the endometrium of MenSCs-treated mice were significantly improved compared to those of PBS-treated mice (P < 0.05), and fibrosis levels were significantly reduced (P < 0.05). Subsequent results revealed that MenSCs treatment significantly promoted angiogenesis in the injured endometrium. Simultaneously, MenSCs enhance the proliferation and antiapoptotic capacity of endometrial cells, which is likely contributed by activating the PI3K/Akt signaling pathway. Further tests also confirmed the chemotaxis of GFP-labeled MenSCs towards the injured uterus. Consequently, MenSCs treatment significantly improved the pregnant mice and the number of embryos in pregnant mice. This study confirmed the superior improvements of MenSCs transplantation on the injured endometrium and uncovered the potential therapeutic mechanism, which provides a promising alternative for patients with serious endometrial injury..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:30 |
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| Enthalten in: |
Reproductive sciences - 30(2023), 11 vom: 12. Juni, Seite 3325-3338 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhang, Shenghui [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| Themen: |
Angiogenesis |
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| Anmerkungen: |
© The Author(s), under exclusive licence to Society for Reproductive Investigation 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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| doi: |
10.1007/s43032-023-01282-0 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Abstract Endometrial injury is one of the leading causes of female infertility and is caused by intrauterine surgery, endometrial infection, repeated abortion, or genital tuberculosis. Currently, there is little effective treatment to restore the fertility of patients with severe intrauterine adhesions and thin endometrium. Recent studies have confirmed the promising therapeutic effects of mesenchymal stem cell transplantation on various diseases with definite tissue injury. The aim of this study is to investigate the improvements of menstrual blood-derived endometrial stem cells (MenSCs) transplantation on functional restoration in the endometrium of mouse model. Therefore, ethanol-induced endometrial injury mouse models were randomly divided into two groups: the PBS-treated group, and the MenSCs-treated group. As expected, the endometrial thickness and gland number in the endometrium of MenSCs-treated mice were significantly improved compared to those of PBS-treated mice (P < 0.05), and fibrosis levels were significantly reduced (P < 0.05). Subsequent results revealed that MenSCs treatment significantly promoted angiogenesis in the injured endometrium. Simultaneously, MenSCs enhance the proliferation and antiapoptotic capacity of endometrial cells, which is likely contributed by activating the PI3K/Akt signaling pathway. Further tests also confirmed the chemotaxis of GFP-labeled MenSCs towards the injured uterus. Consequently, MenSCs treatment significantly improved the pregnant mice and the number of embryos in pregnant mice. This study confirmed the superior improvements of MenSCs transplantation on the injured endometrium and uncovered the potential therapeutic mechanism, which provides a promising alternative for patients with serious endometrial injury. | ||
| 650 | 4 | |a Endometrial injury |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Menstrual blood-derived endometrial stem cells |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Intrauterine adhesions |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Thin endometrium |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Angiogenesis |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Immunomodulation |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a PI3K/Akt signaling pathway |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Zhang, Ruiyun |4 aut | |
| 700 | 1 | |a Yin, Xiyao |4 aut | |
| 700 | 1 | |a Lu, Yuyu |4 aut | |
| 700 | 1 | |a Cheng, Hongbin |4 aut | |
| 700 | 1 | |a Pan, Ying |4 aut | |
| 700 | 1 | |a Liu, Yanli |0 (orcid)0000-0003-0886-8907 |4 aut | |
| 700 | 1 | |a Lin, Juntang |4 aut | |
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